慢性创伤性中的p16和MGMT甲基化:慢性机械刺激和口腔致癌之间存在可信的联系?
Jeronimo Pablo Lazos1, Marcela Hernandez Rios2, Jessica Astorga2
1Oral Medicine Department, Dentistry College, Universidad Nacional de Córdoba, Córdoba, Argentina.
概括
慢性机械刺激 (CMI) 在口腔组织中显著增加p16和MGMT基因甲基化. 通过减少这些表观遗传改变,管理CMI可能有助于预防口腔癌.
科学领域:
- 口腔病理学 口腔病理学
- 癌症表观遗传学 癌症表观遗传学
- 分子生物学分子生物学
背景情况:
- 慢性机械刺激 (CMI) 是口腔癌的一个可疑风险因素.
- 表观遗传变化,如DNA甲基化,是癌症发展的早期事件.
- CMI可能会诱导这些表观遗传变化,促使对慢性创伤性 (CTU) 中的p16和MGMT甲基化进行调查.
研究的目的:
- 调查和描述患有慢性创伤性 (CTU) 的口腔粘膜中p16和MGMT基因的甲基化状态.
主要方法:
- 一项涉及27个人的病例控制分裂口腔研究.
- 皮细胞学被用来收集来自CTU和逆侧正常粘膜的样本.
- 用qPCR和测序分析了p16和MGMT促进体甲基化的DNA.
主要成果:
- 患者的平均年龄为59.1岁.
- 与正常的粘膜相比,在CTU中观察到p16 (85%对20%) 和MGMT (80%对24%) 的甲基化显著更高 (分别为p < 0.0001和p < 0.0005).
结论:
- 持续暴露于CMI与口腔粘膜中p16和MGMT甲基化增加有关.
- 管理机械损伤因素可能对预防口腔状细胞癌 (OSCC) 至关重要.
- 皮细胞学和分口设计是研究CMI中的生物标志物的可行工具,尽管需要进一步研究CMI在口腔癌发生中的作用.
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