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Updated: Feb 19, 2026

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Published on: April 5, 2019

[专注于评估肺炎球菌疫苗反应]

Guillaume Petit¹, Camillia Belhoul¹, Antoine Dinard¹

¹Université Paris Cité, Faculté de Médecine, AP-HP-Centre Université de Paris, Hôpital Cochin, Service d'immunologie biologique, 75679 Paris, France.

Annales de biologie clinique

|February 17, 2026

概括

肺炎球菌合疫苗 (PCV) 对侵袭性感染提供了更好的保护,特别是在儿童中. 评估疫苗反应需要通过ELISA测量特定的IgG,并通过opsonophagocytosis (OPA) 测试测量抗体功能,以获得最佳的公共卫生策略.

关键词:

艾丽莎·伊丽莎·伊丽莎这是OPA OPA.肺炎球菌疫苗肺炎球菌疫苗接种疫苗的反应反应.

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Last Updated: Feb 19, 2026

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Published on: April 5, 2019

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction

Published on: February 24, 2014

Experimental Human Pneumococcal Carriage

Experimental Human Pneumococcal Carriage

Published on: February 15, 2013

科学领域:

免疫学免疫学免疫学
微生物学微生物学
疫苗学疫苗学疫苗学

背景情况:

肺炎链球菌 (Streptococcus pneumoniae) 导致具有高发病率和死亡率的侵入性感染,特别是在脆弱人群中.
细菌多糖囊是肺炎球菌疫苗的关键毒性因素和标,血清型决定了疫苗的疗效.
现有的疫苗包括PPV-23和合疫苗 (PCV),PCV-21代表了最新一代,提供更广泛的血清型覆盖.

研究的目的:

概述世界卫生组织 (WHO) 推的评估肺炎球菌疫苗反应的方法.
详细说明使用ELISA和OPA测试的保护性免疫的确定的值.
为了强调需要更新的疫苗接种建议和扩大响应评估,由于疫苗流行病学的发展.

主要方法:

使用酶相关免疫吸收试验 (ELISA) 量化血清型特异性IgG抗体.
通过opsonophagocytosis (OPA) 试验评估抗体功能.
定义了ELISA (1微克/毫升在成年人中,0.35微克/毫升在儿童中) 和OPA (超过实验室定义的LOQ) 的保护值.

主要成果:

当至少70%的测试血清型 (50%的儿童) 达到ELISA值时,可以获得保护性疫苗免疫力.
如果对至少70%的血清型的opsonization指数超过量化极限 (LOQ),则OPA反应被认为是保护性的.
新的疫苗引入需要定期更新疫苗接种策略和反应评估.

结论:

肺炎球菌疫苗反应的全面评估包括IgG量化 (ELISA) 和功能抗体评估 (OPA).
确定的门为不同年龄组的保护性免疫提供了基准.
调整疫苗推和评估方法至关重要,以应对肺炎球菌疾病流行病学的不断变化.