相关实验视频

Updated: Feb 19, 2026

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

胆固醇在HIV-1感染期间调解GP41线性聚合和增强膜曲率

Junyi Zhao¹, Yang Jiang¹, Li Zhao²

¹State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.

The journal of physical chemistry. B

|February 17, 2026

概括

胆固醇改变了HIV-1 gp41的结构,促进了线性聚合和膜曲. 这种由脂质驱动的机制增强了病毒的进入,并提出了新的融合抑制策略.

科学领域:

生物物理学的生物物理.
病毒学病毒学
膜生物学膜生物学

背景情况:

艾滋病毒-1的进入依赖于信封糖蛋白gp41和膜融合.
胆固醇对于HIV-1感染性至关重要,但其在gp41功能中的作用尚不清楚.

研究的目的:

为了研究胆固醇度如何影响gp41动态和膜重塑.
阐明胆固醇介导的gp41聚类和病毒进入促进的分子机制.

主要方法:

用分子动力学模拟来模拟脂质双层中的gp41行为.
模拟对胆固醇度进行了变化,以观察蛋白质结构和膜曲率的影响.

主要成果:

胆固醇在gp41中诱导了类似雨的形状,有利于线性聚合而不是紧的集群.
胆固醇在gp41集群周围放大了局部膜曲率.
高胆固醇 (≥30%) 驱动形成高度曲的膜结构,增加gp41密度和集群稳定性.

结论:

胆固醇调节gp41相互作用和膜厚度,导致曲率增加和蛋白质聚类.
这些发现为胆固醇在HIV-1入侵中的作用提供了分子框架.
这项研究提出了通过向胆固醇-gp41相互作用来抑制病毒融合的新策略.

更多相关视频

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle GUV Membranes

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle GUV Membranes

Published on: July 28, 2016

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Published on: March 8, 2012

相关概念视频

GPI Anchoring of Proteins in the ER Membrane

GPI Anchoring of Proteins in the ER Membrane

GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Mechanisms of Membrane-bending

Mechanisms of Membrane-bending

The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...