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微尺度热泳传感器用于基于双体结合诱导DNA开关的均和单步蛋白质检测

Han Pang¹, Qiang Zhao²

¹State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

|February 17, 2026

概括

我们开发了一种基于DNA开关的微量热泳 (MST) 传感器,用于敏感和选择性蛋白质检测. 这种方法为生物医学应用提供了一个快速,单步的方法,增强疾病诊断和药物发现.

科学领域:

生物医学工程生物医学工程
分子诊断学分子诊断学
生物化学生物化学

背景情况:

敏感蛋白质检测对于疾病诊断,药物发现和基础研究至关重要.
微尺度热泳 (MST) 是一种新兴的技术,用于实时监测加热下光变化,提供优势,如没有分离,不移动测试,比度测量,快速分析和高吞吐量.

研究的目的:

开发一种单步,选择性和灵敏的微量热泳 (MST) 传感器用于蛋白质检测.
为了在MST分析中利用由aptamer探针组装的DNA开关机制来增强信号生成.

主要方法:

一个DNA开关被设计使用两个aptamer探针,它们与相同的蛋白质标结合,形成一个三明治复合体.
一个aptamer探针被标记为光体;在aptamer终端上的补充序列在蛋白质结合后杂交,形成双重DNA开关.
在MST分析中,在加热时DNA开关的打开产生了与未结合的探针相比不同的MST反应,使得目标蛋白质的检测成为可能.

主要成果:

开发的传感器显示了对目标蛋白的敏感和选择性检测,包括血栓素和血小板衍生生长因子BB (PDGF-BB).
这种DNA开关机制显著提高了局部体探头度,并在加热后MST反应中产生了显著的差异.
该方法通过测量MST信号的显著变化来实现蛋白质检测.

结论:

基于DNA开关的MST传感器为敏感,选择性和快速的蛋白质检测提供了一个有希望的策略.
这种方法适用于各种生物医学应用,包括疾病诊断和药物发现.
开发的传感器克服了传统方法的局限性,提供了高通量和高效的蛋白质分析工具.

关键词:

亚普特美尔 (Aptamer) 是一种药物.生物传感器是一种生物传感器.微尺度的热泳是一种微观的热泳.血小板衍生的生增长因子蛋白质蛋白质是一种蛋白质.血栓激素血栓激素是一种

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