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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

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Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
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使用TT-TSS-seq绘制和量化新生的转录开始站点.

Eleanor Elgood Hunt1, Claudia Vivori1, Richard Mitter1

  • 1The Francis Crick Institute, London, NW1 1AT, United Kingdom.

Genome research
|February 17, 2026
PubMed
概括

我们开发了TT-TSS-seq来绘制新生RNA的转录起点. 这种方法准确量化了启动部位,揭示了对基因调节和不稳定的转录的洞察力.

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 基因法规 基因法规

背景情况:

  • 转录启动是一个复杂的,受监管的过程,决定了基因表达.
  • 从新生的RNA中精确地映射转录起点 (TSSs),对于理解转录动态至关重要.
  • 现有的方法在灵敏度和准确性方面面临挑战,特别是对于不稳定的转录.

研究的目的:

  • 开发和验证一种敏感的方法,用于从新生的RNA绘制和量化转录启动位点.
  • 为了提高低输入RNA样本的转录开始部位测序 (TSS-seq) 的准确性和灵敏性.
  • 调查转录启动模式,包括使用多个TSS和检测不稳定的转录.

主要方法:

  • 结合过渡转录组序列与优化转录开始部位序列 (TT-TSS-seq).
  • 精制的酶反应用于切割和RNA结合以提高灵敏度.
  • 嵌入了具有独特分子标识符 (UMI) 和条形码的5'寡核酸,用于准确的量化和复杂化.

主要成果:

  • 通过使用TT-TSS-seq.成功地绘制和量化了来自新生转录的转录启动位点.
  • 证明了该方法能够检测不稳定的转录的转录启动,例如增强器RNAs.
  • 揭示了很大一部分基因利用多个转录启动位点,往往导致单个稳定的转录.

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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
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相关实验视频

Last Updated: Feb 19, 2026

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结论:

  • TT-TSS-seq提供了转录启动地点的精确映射和量化.
  • 该方法为转录动态和基因调控提供了宝贵的见解.
  • TT-TSS-seq扩展了研究基因表达在初始水平的可用工具.