在小鼠中,前肢相关的M2-M1皮质皮质通路的电路组织
Louis Richevaux1, Rita Fischer2, Miraya Baid2
1Department of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA. louis.richevaux@northwestern.edu g-shepherd@northwestern.edu.
概括
二级运动皮层 (M2) 直接激发主要运动皮层 (M1) 皮层脊髓神经元,与帕瓦胺 (PV) 内神经元介导抑制. 这种M2-M1通路通过细胞类型特定的突触连接调节前肢运动控制.
科学领域:
- 神经科学是一个神经科学.
- 发动机控制器的控制器
- 突触性可塑性 突触性可塑性
背景情况:
- 二级 (M2) 和初级 (M1) 运动皮层之间的通信对于前肢运动控制至关重要.
- 了解参与这种皮质皮质路径的特定突触回路是必不可少的.
研究的目的:
- 为了研究M2和M1运动皮层之间的细胞类型特定的突触连接.
- 为了识别影响M1皮质脊髓神经元的刺激和抑制电路.
主要方法:
- 在雄性和雌性小鼠中利用了细胞类型特定的光遗传学和电生理学.
- 从M1层5B皮质脊髓神经元和其他细胞类型进行记录.
- 使用激光扫描突触输入映射 (sCRACM) 进行功能成像.
主要成果:
- M2轴突强烈激发M1皮质脊髓神经元,能够唤起行动潜力,尽管前进抑制.
- 帕尔瓦胺表达 (PV) 内神经元从M2获得直接激发,并通过强大的抑制进行介导.
- 索马托斯塔丁 (SST),VIP和Ndnf内部神经元对M2输入的刺激和抑制反应各异.
结论:
- M2-M1通路通过直接M2激发和PV介导的M1皮质脊髓神经元的抑制来组织.
- 这种电路配置允许M2通过细胞类型特定的连接调节M1皮质脊髓输出.
- 提供了这个自上而下的电机控制路径的详细的突触线路图.
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