相关实验视频

Updated: Feb 19, 2026

Rapid Isolation of the Mitoribosome from HEK Cells

Rapid Isolation of the Mitoribosome from HEK Cells

Published on: October 4, 2018

酵母和人类线粒体核糖体中的bL36m蛋白的功能分歧

Uliana E Piunova¹, Mariia V Baleva¹, Margarita S Lantsova¹

¹Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.

Biochemistry. Biokhimiia

|February 17, 2026

概括

细菌L36蛋白是不必要的,但它的线粒体对应物 (bL36m) 对于酵母菌的生长至关重要. 缺少bL36m会通过破坏细胞染色体c氧化酶合成,损害酵母中的线粒体功能,但在人体细胞中并非如此.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

核糖体蛋白L36 (L36) 是细菌,线粒体和叶绿体核糖体的结构组成部分.
L36稳定了大型核糖体子单元中的关键功能部位,包括基转移酶中心和L7/L12茎.
在细菌和线粒体中,L36在大型核糖体子单元组装的最后一步被纳入.

研究的目的:

研究L36 (bL36m) 的线粒体同类体在蛋白质合成和线粒体功能中的基本性和功能.
为了比较bL36m缺失在Saccharomyces cerevisiae和人类细胞中的影响.

主要方法:

在Saccharomyces cerevisiae和人类细胞中进行基因删除研究.
分析线粒体核糖体组合和功能.
评估线粒体蛋白质合成和呼吸链复合物的活性,特别是细胞染色体c氧化酶.

主要成果:

删除细菌L36基因并没有影响细菌生长或mRNA转化.
在Saccharomyces cerevisiae中,RTC6基因 (编码BL36m) 的删除导致非发酵碳来源的增长受损,这是由于细胞染色体c氧化酶活性降低和线粒体编码子单元的合成中断.
相比之下,人体细胞中bL36m的缺失并没有显著影响线粒体蛋白质合成或核糖体组合,尽管线粒体呼吸减少了,这表明潜在的呼吸链组织问题.

结论:

线粒体L36同类物 (bL36m) 对于酵母生长和适当的线粒体功能至关重要,特别是影响细胞染色体c氧化酶组合.
在酵母和人体细胞之间,bL36m的功能和实质性显著不同.
虽然人体细胞中bL36m的缺失不会阻止线粒体翻译,但它可能会影响呼吸链组织.

关键词:

线粒体中的线粒体.线粒体的翻译关于翻译的规定.

更多相关视频

Author Spotlight: Advancing Techniques and Discoveries in Protein Synthesis and Assembly Through Innovative Mitochondrial Research

Author Spotlight: Advancing Techniques and Discoveries in Protein Synthesis and Assembly Through Innovative Mitochondrial Research

Published on: June 7, 2024

Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae

Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae

Published on: July 19, 2021

相关概念视频

Porin Insertion in the Outer Mitochondrial Membrane

Porin Insertion in the Outer Mitochondrial Membrane

Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane.
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...

Animal Mitochondrial Genetics

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Structure of Porins

Structure of Porins

Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel...