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在番茄中表达CaCCS编码的桑/sorubin合成酶会增加水果中维生素A的含量和产量

Jingwei Fu¹, Denise Tieman¹, Bala Rathinasabapathi¹

¹Horticultural Sciences Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611-0690.

Plant physiology

|February 18, 2026

概括

我们用桑丁/sorubin合成酶 (CaCCS) 改造番茄以积累β-胡卜素 (维生素A) 和胡的类胡卜素. 这种代谢工程策略提高了营养价值,并产生了更健康的西红.

科学领域:

植物生物技术植物生物技术
代谢工程是代谢工程.
营养科学营养科学

背景情况:

胡卜素,包括β-胡卜素 (provitamin A),是具有健康益处的重要植物颜料.
生物强化战略旨在提高主食作物的营养含量.
胡的特定类胡素,桑丁和素,是强大的抗氧化剂.

研究的目的:

调查桑丁/sorubin合成酶 (CaCCS) 在胡卜素生物合成中的作用.
为了工程番茄 (Solanum lycopersicum) 增强β-胡卜素和类胡卜素的积累.
评估CaCCS在改善番茄营养特征和产量方面的潜力.

主要方法:

(Capsicum annuum) 中的CaCCS病毒诱导的基因沉默.
细菌胡卜素生成系统,以比较CaCCS和番茄基烯β-环酶 (LCYB) 的活性.
在野生类型和突变番茄系 (Micro-Tom' WT和pyp1-1) 中,CaCCS的构成表达.
控制交叉将CaCCS引入选定的西红杂交品种以进行杂交发展.

主要成果:

胡中的CaCCS沉默降低了β-胡卜素的合成和改变了胡卜素的配置.
在细菌中,CaCCS表现出优异的烯循环和独特的桑合成.
过度表达CaCCS的西红显示了桑丁,索鲁宾,β-胡卜素和桑托菲尔的增加.
CaCCS表达增加了水果产量,挥发性物质,氨基酸,和酸,同时减少了糖.
来自CaCCS的番茄杂交品符合维生素A的饮食建议,适度消费.

结论:

CaCCS是莱科循环化和类胡卜素生物合成的关键酶.
过度表达CaCCS是一种强有力的策略,可以用维生素A和其他有益的化合物来生物强化番茄.
桑托菲尔化似乎促进了西红果的总体胡卜素积累.
转基因番茄提供了一种可行的方法来对抗维生素A缺乏症,并提高饮食中的植物化学物质摄入量.

关键词:

这是Solanum lycopersicum中的一个.贝塔胡卜素是一种β-胡卜素.卡普桑提因 (Capsanthin) 是一种含有卡普桑提因的药物.胡卜素是一种胡卜素.果子的收益率是可以获得的.代谢工程是代谢工程.合成生物学合成生物学桑托菲尔 (Xanthophyll) Ester 是一种类型的植物.

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