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Updated: Feb 19, 2026

Cryo-EM and Single-Particle Analysis with Scipion
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Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

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将MD模拟的多切片投影与CryoEM进行比较,暴露了结构预测错误

Arshad Mohammed1, James Lincoff2, Andrew Natale3

  • 1Bay Area Institute of Science, Altos Labs, Redwood City, CA 94065, USA; Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94158, USA.

Biophysical journal
|February 18, 2026
PubMed
概括

分子动力学 (MD) 模拟可以预测生物分子运动和解释冷电子显微镜 (cryoEM) 图像. 然而,目前的MD力场需要精细化以获得准确的膜动力学,特别是复杂的脂质组成.

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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