相关实验视频

Updated: Feb 19, 2026

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

聚合后在室温或2-10°C下存储14天的聚合剩余血清中不同生物化学参数的稳定性

Maja Zabel¹, Thomas Perkmann¹, Patrick Mucher¹

¹Department of Laboratory Medicine, 27271 Medical University of Vienna , Vienna, Austria.

Clinical chemistry and laboratory medicine

|February 18, 2026

概括

聚合的剩余血清对大多数生化分析物具有良好的稳定性,特别是冷藏时. 这使得它们成为外部质量评估 (EQA) 计划的实用资源,如果及时分析.

科学领域:

临床化学临床化学
实验室医学实验室医学
生物化学生物化学

背景情况:

诊断实验室产生大量的剩余血清样本.
利用这些剩余样本可以为实验室测试提供成本效益和可持续的资源.
评估这些聚合样本中分析物的稳定性对于它们在质量保证计划中的应用至关重要.

研究的目的:

为了评估 48 种生物化学分析物的稳定性,在聚合的剩余血清样本中.
在不同的存储条件下评估分析物的稳定性:室温 (RT) 和冷藏 (2-10°C) 最长14天.
确定存储效应在独立血清池中的一致性,以进行外部质量评估 (EQA) 的适用性.

主要方法:

110个残留血清样本被组合在一起,并存储在RT和2-10°C.
分析剂度在0,1,2,3,4,7和14天使用罗氏Cobas和DiaSorin Liaison平台进行测量.
与基线的百分比偏差与最大允许差异进行了比较,并评估了池间变异性.

主要成果:

在冷藏下,只有六种分析物在第14天超过稳定性极限;三种 (alanine aminotransferase,乳酸脱酶,1,25(OH) 2维生素D) 在七天内显示不稳定.
在室温下,48种分析物中的38种在至少4天内保持稳定.
池间变异性在池间基本一致,这表明大多数分析物的矩阵独立降解模式.

结论:

聚合的剩余血清对大多数生物化学分析物具有可接受的稳定性,特别是在冷藏条件下储存时.
在独立池中保持一致的储存效应支持使用这些样本的可重复性.
在适当的时间范围内分析时,聚合的剩余血清是外部质量评估 (EQA) 的可行和实用的资源.

关键词:

生物化学分析剂生物化学分析剂剩下的剩余的剩余的剩余聚合血清的综合血清.在分析前进行预分析.稳定的稳定性稳定的稳定性

更多相关视频

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

Published on: June 11, 2018

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Published on: March 4, 2017

相关概念视频

Drug Product Stability

Drug Product Stability

The long-term stability of drug products is critical to ensuring their quality, safety, and effectiveness over time. Stability directly influences a product's ability to maintain its intended characteristics, ensuring it performs as expected during its intended shelf life. Key attributes such as drug potency, impurities, dissolution, and other physicochemical measures of performance are tested to assess stability. These parameters indicate how well the product retains its quality over time and...

Sample Handling

Sample Handling

Transportation of samples from the collection point to the laboratory, as well as storage and preservation techniques, are crucial for maintaining sample integrity and ensuring accurate and reliable test results.
Samples should be transported carefully from collection points to the laboratory. They should be properly sealed and clearly labeled to prevent cross-contamination. To preserve the sample integrity, optimal temperature conditions during transport are essential. This could involve using...