一种基于同位素稀释-液体色谱-并联质谱的候选参考测量程序,用于测量人体血清和血中的量化
Elie Fux1, Rupert Schmid1, Neeraj Singh1
1Roche Diagnostics GmbH, Penzberg, Germany.
Clinical chemistry and laboratory medicine
|February 18, 2026
概括
一种新的参考测量程序 (RMP) 准确地使用同位素稀释-液态色谱-并联质谱法 (ID-LC-MS/MS) 量化血清和血中的. 这种方法确保了临床诊断和测试标准化的可靠结果.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 准确的丸激素量化对于诊断各种疾病至关重要.
- 现有的方法可能缺乏可靠标准化所需的精度和可追溯性.
研究的目的:
- 开发和验证一种用于测量的候选参考测量程序 (RMP).
- 通过使用初级参考材料,确保SI单位的可追溯性.
- 建立一种可靠的人类血清和血分析方法.
主要方法:
- 同位素稀释-液体色谱-并联质谱法 (ID-LC-MS/MS) 采用二维心脏切割方法.
- 固体相提取 (SPE) 用于样品清理.
- 根据当前指南进行验证,包括选择性,矩阵效应,精度,准确性和不确定性评估.
主要成果:
- 量化范围:27.7 pmol/L到62.4 nmol/L,没有干扰.
- 高精度,中等精度≤3.1%,可重复性为1.4-1.9%.
- 低偏差 (-1.2%至3.0%) 和测量不确定性 (≤2.9%扩展到目标值).
结论:
- 开发的RMP表现出对的高分析性能.
- 该方法适用于常规测试标准化.
- 它为临床样本评估提供准确和精确的结果.
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