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患有败血症风险的患者的外周单核细胞代谢受损:一个队列研究
Velma Herwanto1,2, Ya Wang2, Maryam Shojaei2
1Universitas Tarumanagara, Faculty of Medicine, Jakarta, Indonesia.
概括
免疫细胞代谢甚至在早期感染中受损,在败血症发生之前. 这种早期的线粒体功能障碍和氧化应激可以作为免疫功能障碍和败血症风险的关键生物标志物.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞的新陈代谢
- 败血症病理生理学病理生理学
背景情况:
- 败血症的进展涉及失调的免疫反应和受损的细胞代谢.
- 大多数研究都集中在晚期败血症上,早期的代谢变化没有得到充分的研究.
研究的目的:
- 为了调查免疫细胞在无并发性感染期间的代谢障碍,在败血症发作之前.
- 评估早期代谢功能障碍是否可能表明免疫功能障碍和败血症进展风险.
主要方法:
- 招募的患者有败血症 (n=40),无并发性感染 (n=27) 和健康的志愿者 (n=20).
- 分析了全血样本的基因表达,细胞因子水平和免疫细胞代谢功能 (线粒体呼吸,氧化应激,亡).
主要成果:
- 与对照组相比,免疫细胞在没有并发症的感染和败血症组中显示出线粒体呼吸功能受损.
- 在不复杂的感染中观察到线粒体氧化应激增加,在败血症中进一步升高.
- 在败血症中观察到基因表达变化 (BCL2,BBC3),但不是无并发性感染;亡水平没有显著差异.
结论:
- 免疫细胞代谢功能障碍在败血症临床表现之前的不复杂感染中是明显的.
- 早期的线粒体功能障碍和氧化应激是免疫功能障碍和败血症风险的潜在早期生物标志物.
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