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使用CRISPR/Cas9系统,设计多个模块,以增强Bacillus subtilis中的红细胞纤维素降解能力

Zhiwei Wang^1,2, Gongwei Liu^1,3, Zhongming Meng¹

¹College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.

Synthetic and systems biotechnology

|February 18, 2026

概括

工程 Bacillus subtilis 菌株现在拥有完整的细胞酶系统,可以有效降解纤维素. 这一突破增强了生物质转化,为工业应用提供了一个高性能平台.

科学领域:

生物技术是生物技术.
合成生物学合成生物学
酵素工程是什么意思酵素工程

背景情况:

野生型Bacillus subtilis菌株由于细胞酶活性不完整,在线蛋白纤维素分解效率有限.
开发强大的微生物平台用于生物质转化对于可持续的工业过程至关重要.

研究的目的:

通过使用先进的基因组编辑技术,设计Bacillus subtilis具有完整和高效的细胞酶系统.
为了增强细菌细菌的降解能力,为lignocellulosic生物质.

主要方法:

利用CRISPR/Cas9进行精确的基因组编辑,用于细菌168.
针对细胞酶基因,优化表达录音带,包括信号,终结器和整合点.
构建了具有多个集成细胞酶基因的菌株,并通过编码复合细胞酶系统的等离子体进行转换.

主要成果:

在工程菌株中实现了内葡萄糖酶活性增加的16.29倍.
成功表达和优化双功能细胞质,达到高的外葡萄糖酶和β-葡萄糖酶活性.
开发了BSK3P2C菌株,其细胞酶活性峰值为533.16 U/mL (内葡萄糖酶),2959.83 U/mL (外葡萄糖酶) 和2829.61 U/mL (β-葡萄糖酶).
显示小麦成分 (血纤维素,NDF,ADF) 的显著减少,并证实了广泛的纤维素降解.

结论:

建立了一个高性能Bacillus subtilis平台,配备了完整的细胞酶系统.
工程菌株显示出有效转化纤维素生物质的巨大潜力.
这项工作为工业纤维素降解和利用提供了强大的微生物解决方案.

关键词:

这种细菌是 Bacillus subtilis.这就是CRISPR/Cas9的作用.细胞质细胞质细胞质.表达方式优化表达方式优化红纤维素的生物降解

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