三酸诱导了水生胚胎细胞中的协调DNA甲基化和基因表达变化
Logan S Germain1, Benjamin P Ott2, Charles C T Hindmarch1,2
1Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, K7L 3N6, Canada.
Environmental epigenetics
|February 18, 2026
概括
三酸 (TPHP) 暴露会改变鱼细胞中的基因表达和DNA甲基化. 这项研究揭示了与TPHP相关的表观遗传变化.
科学领域:
- 环境毒理学环境毒理学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 发展性毒性 发展性毒性
背景情况:
- 三酸 (TPHP) 是一种广泛存在的环境污染物.
- 暴露于TPHP与各种毒理效应有关,包括内分泌和代谢干扰.
- 表观遗传机制可能是TPHP多种毒性结果的基础.
研究的目的:
- 调查一个永生钢头鱼细胞系 (STE-137) 中对TPHP暴露的协调转录和表观遗传反应.
- 识别与不良结果途径相关的分子启动事件.
主要方法:
- 暴露STE-137细胞TPHP (0或80μM) 24小时.
- 用于转录概况的RNA测序 (RNA-seq).
- 全基因组二硫酸盐测序 (WGBS) 用于甲基组分析.
主要成果:
- 暴露于TPHP显著改变了1622个基因,丰富了DNA复制,修复,细胞循环,内分泌信号和脂质代谢途径.
- 差异甲基化分析确定了382个差异甲基化区域 (DMR),主要是在基因体内超甲基化.
- 转录基因和表观基因数据的整合揭示了9个具有同时表达和甲基化变化的基因,包括表观基因调节者的改变.
结论:
- 暴露于TPHP会导致水生细胞的显著转录和表观遗传变化.
- 表观遗传修饰,特别是DNA甲基化变化,是TPHP毒性的关键分子事件.
- 表观遗传终点对于评估发育性毒性和了解TPHP的不良结果途径至关重要.
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