一个高效的同热纳米检测平台,将CRISPR/Cas技术用于检测circRNA
Kexin Sun1, Haizhen Wu1,2,3
1State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, China. wuhzh@ecust.edu.cn.
The Analyst
|February 18, 2026
概括
这项研究引入了一种新的纳米检测平台,用于早期三阴性乳腺癌 (TNBC) 诊断,使用CRISPR/Cas技术识别circRNA生物标志物circCD44. 该平台提高了灵敏度和特异性,以改善TNBC检测.
科学领域:
- 生物技术是生物技术.
- 分子诊断学 分子诊断
- 在瘤学瘤学.
背景情况:
- 三阴性乳腺癌 (TNBC) 是一种具有不良预后和具有挑战性的早期检测的侵袭性亚型,原因是其转移和扩散率高.
- 通过生物标志物的TNBC早期诊断对于降低死亡率至关重要.
- 循环RNAs,特别是circCD44,是TNBC的新兴生物标志物.
研究的目的:
- 为TNBC生物标志物circCD44.4设计和验证一个敏感的纳米检测平台.
- 为了利用CRISPR/Cas系统和同热放大,提高检测能力.
- 为早期TNBC诊断提供一种新的策略.
主要方法:
- 开发一个纳米检测平台,集成CRISPR/Cas系统与异热放大.
- 利用功能性核酸分子进行circCD44识别.
- 采用Klenow(3'-5' exo-) 和Cas9n的双信号放大,进一步增强了Cas12a和免疫磁珠.
主要成果:
- 在检测灵敏度方面实现了1.73倍的增强.
- 为circCD44.4建立了从1 pM到100 nM的线性检测范围.
- 显示了95.1 fM的低检测极限 (LOD),与其他五种生物标志物相比具有良好的特异性.
结论:
- 开发的基于CRISPR/Cas的纳米检测平台为circCD44检测提供了一种高度敏感和特定的方法.
- 这个平台代表了早期诊断三阴性乳腺癌的重大进步.
- 该策略有可能用于诊断由特定RNA生物标志物特征的其他疾病.
更多相关视频
10:16Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
Published on: August 16, 2024
2.3K
07:46On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System
Published on: July 18, 2025
1.0K
相关概念视频
01:59CRISPR
58.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.1K
02:53CRISPR and crRNAs
19.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.3K
01:28CRISPR/Cas9 Genome Editing
2.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.1K
01:23The Antiviral System of Bacteria and Archaea: CRISPR
783
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
783