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3D8 scFv的结构引导工程提高了稳定性和抗病毒潜力

Kwang-Ji Oh^1,2, Mee-Hyang Jeon¹, Quynh Xuan Thi Luong³

¹R&D Center, Novelgen Co., Ltd., Yeongtong-gu, Suwon, Republic of Korea.

Biotechnology and bioengineering

|February 18, 2026

概括

改造的抗体碎片 (3D8 scFv) 显示稳定性和产量有所改善. 这些增强型变种保留了对人类冠状病毒OC43的抗病毒活性,为治疗开发铺平了道路.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
结构生物学结构生物学

背景情况:

再组合抗体碎片,如3D8单链可变碎片 (3D8 scFv),由于其抗病毒活性,具有治疗潜力.
不稳定性问题,如溶解度低和聚合,限制了3D8 scFv的临床应用.

研究的目的:

为了提高3D8 scFv的稳定性和生产,使用基于结构的in silico建模和突变发生.
评估工程变异的抗病毒疗效和细胞毒性.

主要方法:

在模拟中,在3D8 scFv.中识别脆弱区域.
局部定向突变发生,以产生单点和双点突变.
在大肠杆菌,SDS-PAGE和尺寸排除色谱中的表达,以评估稳定性和聚合性.
对人类冠状病毒的核酶活性测定和抗病毒测定 OC43.3.

主要成果:

一些突变,包括Y101P和S31W;Y101P,表现出更好的单体稳定性和减少聚合.
突变Y101P和S31W;Y101P显示显著增强表达产量.
工程变体保留了核酶活性,并有效抑制了人类冠状病毒OC43复制.
对测试的突变体没有观察到显著的细胞毒性.

结论:

基于结构的突变发生是一种有效的策略,可以克服单链可变碎片分子的不稳定性.
工程3D8 scFv变种显示出作为稳定,高度表达的抗病毒剂的承诺.
这些发现支持3D8 scFv在工业和治疗应用中的进步.

关键词:

3D8 scFvv 在线观看抗病毒活动的抗病毒活性.蛋白质的稳定性蛋白质的稳定性有理性的突变发生.治疗工程的治疗工程.

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