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通过c-di-GMP调进行生物膜工程,可以提高Acidithiobacillus ferrooxidans中的生物溶解效率和耐受性

Xi Han¹, Yidan Hu², Yanbo Yue²

¹The Key Laboratory of Water and Sediment Sciences, College of Environmental Sciences and Engineering, Peking University, Beijing, China.

Applied and environmental microbiology

|February 18, 2026

概括

经过工程设计的Acidithiobacillus铁氧化物与调制的周期性迪加尼酸单酸盐 (c-di-GMP) 信号显示了增强的生物膜形成,生物溶解效率和耐受性. 这种合成生物学方法为从采矿废物中回收金属提供了一个可持续的战略.

科学领域:

微生物学微生物学
生物技术是生物技术.
环境科学环境科学

背景情况:

生物炼是传统金的可持续替代品,但面临着低效率和重金属抑制等挑战.
铁氧化酸菌 (Acidithiobacillus ferrooxidans) 是一种关键的生物溶解微生物,但其工业应用受到生物膜形成和金属耐受性限制.
目前的基因工程策略通常针对单一的特征,限制了它们对复杂的生物训练挑战的有效性.

研究的目的:

通过调节细胞内 bis(3'-5') - 循环二元格瓦诺辛单酸盐 (c-di-GMP) 信号传输来设计Acidithiobacillus铁氧化物.
为了增强生物膜的形成,生物溶解效率,以及甲基铁氧化物中的耐受性.
为环境友好的生物化和尾矿回收利用提供合成生物学战略.

主要方法:

迪加尼酸环酶基因 (AFE_1379,AFE_0053,AFE_1373) 的过度表达会增加A. ferrooxidans中的细胞内c-di-GMP水平.
创造了具有不同c-di-GMP度的工程菌株S-222,S-306和S-651.
评估不同条件下的生长,生物膜组成 (富含细胞和富含多糖),耐受性,虫沉和生物溶解性能.
转录组分析以了解特定菌株的基因表达模式.

主要成果:

改造后的菌株显示细胞内c-di-GMP水平高1.7至5倍.
菌株S-306 (中间c-di-GMP) 通过富含细胞染色体的生物膜表现出增强的生物炼效率.
菌株S-651 (高c-di-GMP) 通过富含多糖的生物膜显示出更好的耐受性和生物溶解性能.
调节c-di-GMP水平重新编程生物膜矩阵,以改善细胞外电子吸收和金属电阻.

结论:

调整细胞内c-di-GMP水平是一种有效的合成生物学策略,可以增强A. ferrooxidans的生物浸透能力.
工程菌株表现出改善的生物膜特性,从而提高了生物溶解效率和重金属耐受性.
这种方法为从低档矿石和采矿尾矿中可持续生产金属提供了一种多功能生物技术途径.

关键词:

酸性西托巴西勒斯铁氧化物对的耐药性耐的耐药性生物膜矩阵的生物膜.生物教学就是生物教学.bis(3'-5') - 循环二元格瓦诺辛单酸盐 (c-di-GMP) 的方法基因工程是基因工程,是基因工程.

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