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Updated: Feb 20, 2026

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
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催化 pKa 通过遗传代码扩展在水解金属酶中的减弱

Benjamin P Manser1, Alexandria Deliz Liang1

  • 1Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.

Biochemistry
|February 18, 2026
PubMed
概括

基因代码扩展设计了一种金属酶,以增强酸性活性. 突变一个关键的胺残留物,在较低的pH值下提高了酶耐受性和催化效率.

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