相关实验视频
Updated: Feb 20, 2026
09:56
Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
Published on: September 6, 2019
7.3K
在Bacillus amyloliquefaciens中编程低分子量氨酸生物合成通过自诱导分子开关
Qian Zhong1,2, Zhen Li1,2, Waibin Duan1,2
1College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, P. R. China.
ACS synthetic biology
|February 18, 2026
概括
研究人员在Bacillus amyloliquefaciens中设计了一种自我诱导的分子开关,用于高效的氨酸 (HA) 生物合成. 这种新的系统可在不需要诱导剂的情况下,高效地提高HA的生产成本.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 微生物生物技术 微生物生物技术
背景情况:
- 动态代谢调节对于将代谢流转向有价值的化合物生物合成至关重要.
- 控制新陈代谢通路的现有方法通常需要外部诱导剂,增加成本.
- 氨基基菌是工业生物生产的关键宿主.
研究的目的:
- 设计和实施一种可自诱导,无诱导剂的分子开关,用于增强生物产品合成.
- 为了改善氨酸 (HA) 产量,并尽量减少Bacillus amyloliquefaciens中的副产品形成.
- 开发适用于各种高价值化合物的生物合成的多功能平台.
主要方法:
- 集成了一种来自Pantoea藻类的PaSspB-ssrA*蛋白质溶解系统 (OFF模块) 与一个DegU-RapG-PhrG定数感应 (QS) 模块 (ON模块).
- 利用PaSspB-ssrA*系统有效降解ssrA*标记的蛋白质.
- 采用DegU激活的P_ispA促进剂用于细胞密度依赖的基因表达,将ssrA*标签与糖解基因 (pfkA,fruA) 融合,并在工程菌株中共同表达PaSspB和SthHL.
主要成果:
- 在针对性降解ssrA*标记蛋白质方面获得了86%的效率.
- 在工程化Bacillus amyloliquefaciens中成功生产了13.5 ± 0.19 g/L的低分子量氨酸 (2.89 × 10^4 Da).
- 通过减弱的糖溶性流体证明了减少副产品的积累.
结论:
- 开发的自诱导分子开关为HA生物合成提供了一个自主且具有成本效益的平台.
- 该系统显示出广泛适用于高价值生物制品的高效合成.
- 这种方法为工业生物技术的微生物细胞工厂设计提供了重大进步.
相关概念视频
01:24Biosynthesis in Bacteria
783
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
783
01:28Gene Regulation in Microbial Communities: Quorum Sensing
742
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
742
01:26Biosynthesis of Polysaccharides
733
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
733