聚乙烯纳米塑料通过细胞骨重塑和表观遗传重编程破坏卵巢发育,特别是在颗粒细胞中
Mengyao Liu1, Shenao Wang2, Weijun Gao3
1Key Laboratory for the Genetics of Development & Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200240, China.
Journal of hazardous materials
|February 18, 2026
概括
聚乙烯纳米塑料 (NP) 通过破坏卵巢细胞和改变基因表达来损害雌性小鼠的生育能力. 这项研究揭示了NP通过细胞骨变化和表观遗传重编程来破坏繁殖,突出了环境健康风险.
科学领域:
- 环境健康 环境健康
- 毒理学 毒理学 毒理学
- 生殖生物学 生殖生物学
背景情况:
- 微塑料和纳米塑料 (M/NPs) 是新兴的环境污染物.
- 有证据表明,M/NPs可以积聚在生殖腺中并影响生育能力,但机制尚不清楚.
研究的目的:
- 研究聚烯微型和纳米塑料 (PS-M/NP) 对雌性小鼠卵巢功能的影响.
- 阐明PS-NP诱导的生殖毒性的细胞和分子机制.
主要方法:
- 在体内和体外暴露模型使用100nm和5μm的青春期雌性小鼠PS-M/NP.
- 单细胞RNA测序 (scRNA-seq) 和ATAC-seq用于分析卵巢细胞反应.
- 在体外测试以确认NP内部化和细胞效应在颗粒细胞 (GCs).
主要成果:
- 100纳米PS-NP引起了尺寸依赖的生殖毒性,包括卵巢尺寸减少和毛囊发育中断.
- scRNA-seq确定了颗粒状细胞作为主要点,具有改变的细胞内通信和FSCN1失调.
- 实验室研究表明,100纳米PS-NP诱导GC细胞周期停止,亡和通过细胞骨重塑和表观遗传变化 (H3K4me3,H3K27ac) 的荷尔蒙功能障碍.
- 确定STAT1是PS-NP诱导的表观遗传和转录变化的关键调节者.
结论:
- 这项研究提供了PS-NP介导的卵巢毒性首个单细胞分辨率图谱.
- 通过破坏细胞骨架和重新编程表观遗传景观,NP 破坏了哺乳动物的繁殖.
- 这些发现突出了与环境M/NP暴露相关的潜在生殖健康风险.
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