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在暴露于化液晶单体的斑马鱼中,线粒体功能障碍和AMPK相关的代谢重编程

Junjie Wang¹, Siying Ma², Siyi Li²

¹Beijing Key Laboratory of Urban Hydrological Cycle and Sponge City Technology, College of Water Sciences, Beijing Normal University, Beijing 100875, China; School of Environment and Resource, Southwest University of Science and Technology, Mianyang, Sichuan 621010, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

Ecotoxicology and environmental safety

|February 18, 2026

概括

化液晶单体 (FLCM) 破坏细胞能量生产,影响线粒体功能和新陈代谢. 这项研究揭示了它们的毒性机制,突出了代谢障碍的潜在风险.

科学领域:

环境毒理学环境毒理学
代谢学代谢学代谢学
线粒体功能障碍线粒体功能障碍

背景情况:

化液晶单体 (FLCM) 是人类和水生环境中检测到的持久电子废物污染物.
对于FLCM代谢毒性的亚细胞机制的了解很少.
了解FLCM的毒性至关重要,因为它们在环境中越来越普遍.

研究的目的:

研究由FLCMs引起的线粒体和代谢干扰.
阐明FLCM诱导的毒性背后的亚细胞机制.
确定受FLCM暴露影响的关键代谢途径.

主要方法:

斑马鱼胚胎和幼虫接触了五种代表性的FLCM.
评估了线粒体呼吸,ATP生产,NAD+/NADH比率和膜潜力.
分析了酶活性 (ICDHc,α-KGDH),基因表达 (pdk2,gls,mt-nd1) 和目标代谢.
使用了KEGG丰富分析和与AMPK抑制剂 (化合物C) 的同时暴露.

主要成果:

环境相关的FLCM度降低了基础呼吸,ATP相关的氧气消耗,NAD+/NADH比率和膜潜力.
观察到ICDHc和α-KGDH活动的补偿上调,表明TCA循环激活.
暴露于FLCM诱导了AMPK相关的代谢转变,由基因表达变化和C化合物逆转证实.
代谢学和KEGG分析显示,碳流转向通过AMPK-谷氨酸-TCA轴转向由氨基酸驱动的阿纳普勒索斯.

结论:

FLCMs作为次致命的线粒体毒素,破坏细胞能量代谢.
AMPK-Glutamate-TCA轴是FLCM诱导的代谢干扰的一个关键机械特征.
由FLCM诱导的代谢重编程可能会增加对代谢障碍的敏感性,这需要进一步调查和环境监测.

关键词:

在AMPK信号传输中.新出现的污染物新出现的污染物能量代谢能量代谢化液晶单体分子的化液晶单体代谢重编程是一种代谢重编程.斑马鱼是一种斑马鱼.

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