相关实验视频

Updated: Feb 20, 2026

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Published on: March 24, 2020

对 styrene 和 styrene-7,8-oxide 基因毒性文献的批判性审查:一个更新

Lynn H Pottenger¹, Marcy Banton², Wade T Barranco³

¹LHP Tox Consult, LLC, Midland, MI, USA.

Journal of environmental science and health. Part C, Toxicology and carcinogenesis

|February 19, 2026

概括

styrene 和 styrene 氧化物在体外表现出突变性潜力. 然而,最近的研究证实,烯不是动物体内的突变原体,也不是人类细胞遗传损伤的原因.

科学领域:

毒理学毒理学毒理学
遗传学是一个遗传学.
职业健康职业健康职业健康

背景情况:

最近对 styrene 和 styrene 氧化物 (SO) 致变性/细胞遗传效应的评估从2019年开始进行了更新.
首次包括关于 styrene/SO 基因毒性的转录学研究.
考虑了初级DNA损伤终点数据.

研究的目的:

批判性地评估最近关于 styrene 和 SO 的突变性和细胞遗传效应的数据.
为了解决烯体内突变性研究中的数据缺口.
更新关于烯在实验动物和人类中的基因毒性证据权重.

主要方法:

关于烯和SO的突变/细胞遗传作用的最近研究的综述.
包括与基因毒性相关的转录组学研究.
考虑主要的DNA损伤终点.
对动物体内致变性和基因毒性研究的分析符合经合组织指导方针.
审查最近关于工作场所烯暴露的流行病学研究.

主要成果:

最近对小鼠和老鼠进行的符合经合组织指导方针的研究表明,在多种组织或骨髓中没有突变性.
最近的重复剂量动物研究对DNA损伤和微核产生了负面结果.
流行病学研究没有提供额外的证据表明烯暴露会导致细胞遗传损伤增加.
在某些条件下,烯和SO在体外表现出致变体或细胞遗传作用.

结论:

没有令人信服的证据表明,烯在实验动物中是一种体内变异原体或克拉斯托根/新原体.
目前的证据不支持工作场所的施烯暴露导致人类细胞遗传损伤的频率增加.
虽然 styrene 和 SO 可以在体外诱导效应,但在动物和人类体内数据没有显示出令人信服的基因毒性证据.

关键词:

在DNA中,添加物是DNA的添加物.造成的DNA损伤是DNA损伤.致变性致变性致变性可持续发展目标12:负责任的消费和生产可持续发展目标8:体面的工作和经济增长这就是克拉斯托基性 (clastogenicity).翻译学翻译学翻译学翻译学

更多相关视频

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

Published on: August 1, 2015

相关概念视频

Mutagenicity and Carcinogenicity

Mutagenicity and Carcinogenicity

Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...

Toxic Reactions: Overview

Toxic Reactions: Overview

When toxic substances penetrate the human body, they disseminate to various tissues, undergoing metabolic changes. This process yields reactive metabolites that may covalently bind with specific target molecules, resulting in toxicity.
Toxicity falls into two primary categories: local and systemic.
Local toxicity appears at the exposure site, such as protein denaturation caused by caustic substances.
In contrast, systemic toxicity requires the toxic agent's absorption and distribution,...

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Toxicity Testing in Animals

Toxicity Testing in Animals

Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...

Bioactivation and Tissue Toxicity

Bioactivation and Tissue Toxicity

Bioactivation is a metabolic process that transforms less reactive substances into highly reactive metabolites, initiating tissue toxicity. This transformation can lead to various toxic effects, including carcinogenesis and teratogenesis. Reactive metabolites are classified into two main types: electrophiles and free radicals.Electrophiles are electron-deficient species and are produced primarily by the enzyme cytochrome P-450 during the metabolism of compounds containing carbon, nitrogen, or...

DNA Damage can Stall the Cell Cycle

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...