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Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Updated: Feb 20, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

多层封闭单分子电荷转移激活PRET用于近红外向细胞成像

Zhuo Lei¹, Sai Li¹, Pei-Ao Sun¹

¹College of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin, P. R. China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|February 19, 2026

概括

此摘要是机器生成的。

这项研究引入了一种新的光共振能量转移 (PRET) 系统,使用超分子组件进行先进的生物成像. 该系统实现近红外延迟光,克服了用于增强细胞可视化的传统限制.

关键词:

细胞成像细胞成像转移费用转移费用转移费用转移费用多层次的限制限制.接近红外的延迟光发射.光共振传输能量转移的能量

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Published on: February 8, 2015

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Last Updated: Feb 20, 2026

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科学领域:

超分子化学超分子化学
生物成像是一种生物成像.
材料科学材料科学材料科学

背景情况:

光共振能量转移 (PRET) 通常具有短波长限制.
开发用于近红外 (NIR) 生物成像的高效PRET系统仍然是一个挑战.
超分子组件为先进的光学应用提供可调节的特性.

研究的目的:

设计和描述一种用于高性能PRET的新型超分子组件.
为了实现NIR延迟光发射,提高生物成像能力.
展示该系统在活细胞成像和其他应用中的潜力.

主要方法:

采用二基桥接二衍生物 (G1),库库比特[8]uril (CB[8]) 和β-环极素移植的氨酸 (HACD) 来构建一个量身定制的电荷转移超分子组件.
研究组件的结构转变和能量转移动态.
从甲 (供体) 转化为甲 (接受体) 的PRET与NIR延迟光发射的特征.
评估系统在活细胞标记和检测方面的性能.

主要成果:

一个超分子组件被成功构造,表现出很大的斯托克斯转移 (320 nm) 和高效的能量转移.
该系统实现了单分子PRET,将光扩展到650nm的NIR延迟光,寿命为11.20μs.
在活细胞中证明了成功的NIR通道标记和检测,验证了其生物成像潜力.

结论:

开发的超分子PRET系统克服了传统的波长限制,使高性能NIR生物成像成为可能.
合理的结构定制和封闭工程是推进超分子PRET设计的关键.
这项工作对细胞成像,信息加密和防伪应用具有广泛的意义.