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打破障碍:从X射线结晶学过渡到冷EM进行结构研究

Hassan Zafar1, Kiera L Malone1, Ajit K Singh1

  • 1Department of Pharmacology, Larner College of Medicine, University of Vermont, Burlington, VT 05405, USA.

Acta crystallographica. Section D, Structural biology
|February 19, 2026
PubMed
概括

这项研究详细介绍了向冷电子显微镜 (cryo-EM) 过渡,以研究ATAD2B蛋白. 它为结构生物学中的挑战提供了实际的解决方案,特别是对于大型,灵活的复合体.

关键词:
ATAD2B ATAD2B ATAD2B ATAD2B ATAD2B ATAD2B ATAD2B ATAD2B ATAD2B格罗埃尔 (GroEL) 是一个古老的城市.类域名 类域名冷电子显微镜的使用方法数据处理工作流程.宏分子X射线晶体学 宏分子X射线晶体学建模和改进模型的建设和改进.蛋白质表达和净化过程一个粒子分析分析.结构生物学结构生物学

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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