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用细菌曼纳酶生物工艺可持续生产 prebiot mannooligosaccharides 使用细菌曼纳酶

Monika Rana¹, Aditya Kumar¹, Rahul Warmoota¹

¹Department of Microbiology, Panjab University, Chandigarh, 160014 India.

Indian journal of microbiology

|February 19, 2026

概括

这项研究利用酶优化了粉中曼诺糖 (MOS) 的产生,实现了4.6倍的增加. 由此产生的MOS表现出益生菌性质,使其适合功能性食品.

科学领域:

生物技术是生物技术.
食品科学食品科学食品科学
生物化学生物化学

背景情况:

曼诺酸糖 (MOS) 具有宝贵的功能性质,如前生物和抗氧化作用.
经济和可持续的MOS生产对于广泛应用至关重要.
目前用于MOS生产的方法需要针对大规模使用进行优化.

研究的目的:

标准化使用β-曼酶 (MAN-7) 来从铜粉中增强MOS生产的条件.
描述产生的β-MOS并评估它们的前生物潜力.

主要方法:

使用来自 * Bacillus subtilis * MAN7.7 的β-mannanase 来进行铜粉的酶性水解.
使用活性炭和乙醇处理净化β-MOS.
用光谱和色谱技术对β-MOS进行表征.
通过评估细菌生长促进和对消化条件的抵抗来评估益生菌效应的评估.

主要成果:

实现了MOS产量的4.6倍增长,产量高达560mg/g的铜粉.
纯化的β-MOS,其聚合度 (DP) 为2-6.
通过促进*Lactobacillus* spp.通过促进*Lactobacillus* spp.进行确认的益生菌活性. 增长和抑制肠道病原体.
已证明MOS对消化液的耐药性和缺乏细胞毒性.

结论:

优化的酶条件为从农业废物中生产MOS提供了成本高效的生物工艺.
产生的β-MOS由于其已证明的健康益处,因此适用于功能性食品成分和益生菌剂.
这项研究提供了一种可持续的方法,用于将粉提升为有价值的β-MOS.

关键词:

生物活性的生物活性.科普拉的面粉是可以吃的.功能性食品是一种功能性食品.不易消化的寡糖类.废弃物价值化废弃物价值化废弃物价值化

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