由 Agrococcus sp. 驱动的循环生物经济驱动的胡卜素生产. NP24使用奶酪乳清副产品:过程优化和生物活性评估
Nehad Noby1, Fatma Elsayed2, Mahmoud M Agami3
1Biotechnology Department, Institute of Graduate Studies and Research (IGSR), Alexandria University, Alexandria, Egypt.
Frontiers in microbiology
|February 19, 2026
概括
亚格罗科库斯 (Agrococcus sp.) 是一个有趣的植物. NP24从奶酪乳清中生产了热丁单,这是一个可持续的农业食品废物. 这种天然色素表现出良好的稳定性和强大的抗氧化和抗癌活性,这表明它可以作为食品染色剂使用.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
背景情况:
- 循环经济原则推动农业食品废弃物用于微生物颜料生产的价值化.
- 奶酪乳清是一种易于获得的,富含营养的基质,用于可持续的生物工艺.
研究的目的:
- 为了优化Agrococcus sp.的颜色合成. NP24使用奶酪乳清.
- 描述色素并评估其稳定性和生物活性.
主要方法:
- 使用Box-Behnken设计 (BBD) 的中等优化.
- 通过HPLC-DAD和LC-MS进行颜料识别.
- 颜料稳定性 (pH,温度,光) 和生物活性 (抗氧化剂,抗癌剂,抗菌剂) 的评估.
主要成果:
- 使用优化的媒介 (80%乳清,0.5%,0.97%素,0.5%酵母提取物,0.5%MgSO4) 达到最大的色素产量.
- 被识别为泽丁单 (C14:0) 的颜料,在高达50°C和酸性条件下稳定 (pH 3-5).
- 强大的抗氧化剂 (IC50 = 6μg/mL) 和针对MDA-MB-231和HCT-116细胞系的选择性抗癌活性.
结论:
- 从奶酪乳清中生产NP24类胡卜素的成本效益是可行的.
- 山丁单具有良好的稳定性和显著的生物活性.
- 作为食品中的天然着色剂的潜在应用.
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