在中国出现一种高度毒性的猪流行性腹病毒 (PEDV) G2c亚型:隔离,遗传和病原性特征以及交叉中和抗体反应
Yang-Yang Li1,2, Chuan-Hao Fan2, Hai-Xia Li2
1State Key Laboratory of Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, China, scau.edu.cn.
Transboundary and emerging diseases
|February 19, 2026
概括
猪流行性腹病毒 (PEDV) 的新兴G2c变种显示了尖端蛋白的突变,降低了疫苗的有效性. 需要新的策略来对抗猪中这些高度毒性菌株.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 免疫学 免疫学 免疫学
背景情况:
- 猪流行性腹病毒 (PEDV) 在全球范围内导致小猪的显著死亡率.
- 在PEDV尖峰 (S) 蛋白中发生的突变,特别是在G2c变体中,降低了当前疫苗的疗效.
- 缺乏对新出现的G2c变体的全面研究.
研究的目的:
- 为了隔离和描述一个G2c PEDV菌株.
- 分析G2c变异的遗传学和结构影响.
- 为了评估G2c PEDV的免疫性和病原性,用于疫苗开发.
主要方法:
- 病毒隔离和S基因的测序.
- 对PEDV菌株的遗传学分析.
- 对S蛋白变体的结构建模.
- 血清中和抗体测试. 血清中和抗体测试.
- 在新生猪小猪的致病性评估.
主要成果:
- 隔离了一种G2c PEDV菌株 (AHCZ02);在最近的疫情中,G2c变种占主导地位 (69.57%).
- 确定了四种特定于G2c的S蛋白替代 (N139D,I287M,F345L,L998M),导致了形状变化.
- 基于G2c的反策略显示中和抗体标位明显高于基于S-INDEL的方法和传统疫苗.
- AHCZ02导致小猪在66小时内100%死亡,严重的临床症状和肠道损伤.
结论:
- G2c PEDV 变种具有 S 蛋白修饰,损害了疫苗的疗效.
- 下一代疫苗应该包含G2c特异性抗原.
- 加强病毒学监测对于监测PEDV演变和控制疫情至关重要.
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