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Cell Migration

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Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.

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Updated: Jun 16, 2026

Identification and Dissection of Diverse Mouse Adipose Depots

Identification and Dissection of Diverse Mouse Adipose Depots

Published on: July 12, 2019

一种敏感的记者鼠标模型,用于在体内研究由脂肪细胞衍生的细胞外囊泡.

Didde Riisager Hansen^1,2, Rugivan Sabaratnam^1,2,3, Lasse Bach Steffensen¹

¹Department of Molecular Medicine, University of Southern Denmark, Odense, Denmark.

Journal of extracellular vesicles

|February 19, 2026

概括

研究人员开发了一种敏感的体内记者系统,使用腺相关病毒 (AAV) 载体来跟踪脂肪细胞衍生的细胞外囊泡 (EV). 这个系统揭示了早期高脂肪饮食对小鼠EV分布的影响.

关键词:

CD63 CD63 是一个在CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 克雷复合酶可以重组.电动汽车记者电子汽车记者脂肪组织的脂肪组织.纳米化酶是一种纳米化酶.肥胖肥胖肥胖肥胖肥胖肥胖肥胖肥胖

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Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro

Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro

Published on: December 6, 2025

相关实验视频

Last Updated: Jun 16, 2026

Identification and Dissection of Diverse Mouse Adipose Depots

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Published on: July 12, 2019

A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk

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Published on: November 8, 2019

Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro

Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro

Published on: December 6, 2025

科学领域:

生物化学生物化学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

细胞外囊泡 (EVs) 在体内细胞间通信中起着至关重要的作用.
在生物流体中研究特定细胞类型的EV丰富度和分布存在重大技术挑战.

研究的目的:

开发一种敏感的体内报告系统,用于监测细胞类型特定的EV,重点关注脂肪细胞衍生的EV.
使用开发的报告员系统,调查高脂肪饮食 (HFD) 对脂肪细胞衍生的EV的影响.

主要方法:

生成的腺相关病毒 (AAV) 载体编码Cre-激活的CD63-NanoLuc (CD63-NanoLuc) 和组成的sec-NanoLuc记者.
向小鼠注射AAV以达到脂肪细胞特异性记者表达.
在正常和HFD条件下的小鼠的血,尿液和组织中测量了NanoLuc活性.

主要成果:

这两种报告者的脂肪细胞特异表达被AAV管理成功诱导.
CD63-NanoLuc活性主要在脂肪组织中检测到,而sec-NanoLuc在血和尿液中发现.
在脂肪组织,肺部,脏和尿液中,HFD显著增加了CD63-NanoLuc活性,表明脂肪细胞衍生的EV释放和分布发生了变化.

结论:

CD63-NanoLuc和sec-NanoLuc报告员系统提供了一种敏感的方法,用于在体内监测特定细胞类型的EVs.
该研究表明HFD对脂肪细胞衍生的EV丰度和分布的早期影响,可通过报告系统检测到.
该系统为在各种生理和病理条件下研究电动车提供了有价值的工具.