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从草本提取物中选天然血栓抑制剂,使用酶固定微流体反应器进行选

Xian-Na Wang¹, Ji Zhang¹, Yahui Song¹

¹State Key Laboratory of Natural Medicines and School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, China. weiweitang@cpu.edu.cn.

|February 19, 2026

概括

一种新型的酶固定微流体反应器 (EIMR) 有效地选草本提取物以检测血栓抑制剂. 这种高灵敏度的工具确定了八种潜在的抑制剂,展示了其在从自然来源发现药物的实用性.

科学领域:

生物化学和生物技术
分析化学分析化学
材料科学材料科学材料科学

背景情况:

酶固定微流体反应器 (EIMR) 在体外催化剂和抑制剂选中非常有价值.
从复杂的草本提取物中选潜在的血栓抑制剂需要高效和敏感的平台.

研究的目的:

开发一个由酶固定的微流体反应器 (EIMR),集成到玻璃聚二甲基 (PDMS) 混合微流体芯片 (HMC) 中.
通过使用双模式检测 (光和MALDI MS) 来从复杂的草本提取物中选潜在的血栓抑制剂.

主要方法:

选择性固定血栓在金纳米粒子功能化的多孔片 (AuNPs@FPSF) 涂层的ITO玻璃片上,使用DNA吸收剂TBA15.5进行血栓选择性固定.
在PDMS板中制造微流体反应器,形成混合微流体芯片 (HMC).
双模型检测使用合成光基质进行现场监测,并使用MALDI MS进行量化.

主要成果:

优化的血栓固定和光基质度改善了EIMR的可重现性.
从五种草本提取物中成功鉴定出八种潜在的血栓抑制剂.
使用MALDI MS.确定已识别的化合物的抑制活性.

结论:

开发的EIMR为选酶抑制剂提供了高灵敏度,易于使用和快速响应.
这个平台有助于从复杂的草药来源发现候选酶抑制剂.
EIMR是生物活性化合物的现场查和分析的一个有前途的工具.

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