从发酵产品中分离的潜在益生菌乳酸细菌的隔离,特征和脂质细胞介导的封装
Benay Çolak1, Dilan Barut Yavaş1, Belma Nural Yaman1,2
1Department of Biotechnology and Biosafety, Eskisehir Osmangazi University, Eskisehir, Türkiye.
Current microbiology
|February 19, 2026
概括
牛乳杆菌delbrueckii亚种. 这种细菌. 布尔加里克斯BC-16是一种来自自制酸奶的强效益生菌,证明了安全性和有效性. 将这种益生菌封装在脂质体中显著提高了它对胃肠道疾病的生存率.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 乳酸细菌 (LAB) 在发酵食品中至关重要,提供骨支持和糖尿病管理等健康益处.
- 益生菌是功能性食品的关键成分,需要保护才能达到最佳效果.
- 这项研究调查了传统发酵产品中的LAB菌株的益生菌潜力.
研究的目的:
- 为了描述LAB菌株的益生菌性质,从自制的酸奶和.
- 为了确定功能性食品应用中最有效的益生菌菌株.
- 为了提高胃肠道稳定性,将选定的益生菌菌株封装在脂质体中.
主要方法:
- 对LAB菌株的隔离和功能特征.
- 细胞毒性测定,抗生素敏感性测试,外聚糖类的产生,以及疏水性测量.
- 选择的益生菌菌株 (L. delbrueckii亚种) 的脂质体封装. 布尔加里克斯BC-16) 和体外胃肠道生存测试.
主要成果:
- 在L.delbrueckii亚种中. 布尔加里克斯BC-16表现出良好的安全性 (低细胞毒性) 和功能特征 (高外聚糖类产量,疏水性).
- 脂质体中的封装效率约为86.8%,封装后过程效率为62.5%.
- 在体外胃肠道挑战后,脂质细胞封装的益生菌显示出明显更高的活力 (6.90 log CFU/mL) 与自由细胞 (6.50 log CFU/mL) 相比.
结论:
- 在L.delbrueckii亚种中. 由于其安全性和功能特性,Bulgaricus BC-16是益生菌应用的有希望的候选者.
- 脂质体封装有效地保护益生菌免受严重的胃肠道疾病,提高它们的稳定性和活力.
- 这项研究突显了脂质体技术在开发具有更好的益生菌功效的高级功能性食品方面的潜力.
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