Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Interference01:23

RNA Interference

24.3K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
24.3K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

6.1K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Generative design and validation of therapeutic peptides for glioblastoma based on a potential target ATP5A.

Briefings in bioinformatics·2026
Same author

GN-Net: A Geometric and Neighborhood-Aware Network for Predicting Intracranial Aneurysm Rupture Risk and Assisting Clinical Decision-Making.

Interdisciplinary sciences, computational life sciences·2025
Same author

Mitochondrial Proteome Reveals Metabolic Tuning by Restricted Insulin Signaling to Promote Longevity in <i>Caenorhabditis elegans</i>.

Biology·2025
Same author

Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cells.

Cell discovery·2025
Same author

Mammalian PIWI-piRNA-target complexes reveal features for broad and efficient target silencing.

Nature structural & molecular biology·2024
Same author

Efficient retrosynthetic planning with MCTS exploration enhanced A<sup>*</sup> search.

Communications chemistry·2024

相关实验视频

Updated: May 1, 2026

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

11.7K

PAIRNet:通过位置感知RNA相互作用建模预测PIWI裂变特异性.

Lin Zeng1, Zhenzhen Li2, Enzhi Shen2

  • 1Center for Cognitive Machines and Computational Health (CMaCH), School of Computer Science, Shanghai Jiao Tong University, Shanghai, China.

PLoS computational biology
|February 19, 2026
PubMed
概括

深度学习框架PAIRNet通过建模导向-目标相互作用,准确地预测PIWI介导的RNA裂变率. 这种计算工具增强了对piRNA沉默的理解,并加速了基因组防御研究.

更多相关视频

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
07:24

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

1.7K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.9K

相关实验视频

Last Updated: May 1, 2026

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

11.7K
Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
07:24

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

1.7K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.9K

科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 通过piRNA引导的RNA分裂,PIWI蛋白对基因组完整性至关重要.
  • Cleave-N'-Seq (CNS-seq) 映射了PIWI的目标,但具有劳动密集型的工作流.
  • 在PIWI准中对序列决定因素的系统探索是有限的.

研究的目的:

  • 开发PAIRNet,这是一个深度学习框架,用于预测PIWI介导的RNA分裂率.
  • 为了建模导向-目标相互作用,考虑几何和序列.
  • 加速对RNA导向基因组防御的机制研究.

主要方法:

  • 开发了PAIRNet,这是一个集生化学见解和计算方法的深度学习框架.
  • 编码的配对状态,不匹配,插入,删除和位置嵌入.
  • 采用混合CNN-变压器架构来优先考虑双重动态.
  • 集成的可解释性模块 (销售权图,反事实分析).

主要成果:

  • 在四个PIWI指导数据集中,PAIRNet准确地预测PIWI介导的RNA分裂率.
  • 与现有方法相比,在PCC方面取得了显著的改进 (34.7%用于MILI,14.6%用于MIWI).
  • 总结了关键的生物学原理,包括对催化残留的严格互补性和3'不匹配容忍度.

结论:

  • PAIRNet将生物化学精度与PIWI目标分析的计算可扩展性相结合.
  • 建立了设计高特异性piRNA沉默工具的路线图.
  • 加快对RNA导向基因组防御的机制研究.