Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Shared Genomic Architecture Between Schizophrenia and Multiple Sclerosis Identifies an Un-Drugged HCAR1 Neuroimmune Checkpoint.

bioRxiv : the preprint server for biology·2026
Same author

State-Dependent Transcriptomic Collapse of the Brain's Lactate and Ketone Thermodynamic Sensors in Schizophrenia.

bioRxiv : the preprint server for biology·2026
Same author

Metabolic Gating and the Evolution of Human Cognitive Plasticity: A Comparative Genomic Analysis.

bioRxiv : the preprint server for biology·2026
Same author

Rational Engineering of the Anthrax Toxin Nanopore Interface for Orthogonal Peptide Classification.

ACS omega·2026
Same author

Peptide properties predict multistate translocation kinetics via protective antigen nanopores.

Biophysical journal·2026
Same author

ADLM Guidance Document on Laboratory Testing for Drugs of Misuse to Support the Emergency Department.

The journal of applied laboratory medicine·2026

相关实验视频

Updated: Jun 19, 2026

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

14.2K

一个动态的炭毒素纳米孔生物传感器,用于高保真单分类.

Jennifer M Colby1, Bryan A Krantz2

  • 1Molecular Toxicology Graduate Program, University of California, Berkeley, California, United States of America.

PLoS computational biology
|February 19, 2026
PubMed
概括

这项研究引入了一种新的纳米孔传感方法,用于实时单分子蛋白质组学. 它准确地识别了复杂混合物中的,而没有平均值,为高级蛋白质组分析铺平了道路.

科学领域:

  • 生物物理学的生物物理.
  • 分析化学 分析化学
  • 生物化学 生物化学

背景情况:

  • 目前用于蛋白质组学的纳米孔传感依赖于集体聚合,限制了复杂样品的分析.
  • 个别的实时识别对于破译异质生物混合物至关重要.

研究的目的:

  • 开发一种从单个转位事件中对的高保真分类方法.
  • 消除了对纳米孔基蛋白质组学组合平均值的需求.
  • 为了实现真正的单分子蛋白质组学,用于复杂混合物分析.

主要方法:

  • 使用了炭毒素保护抗原 (PA) 的纳米孔,具有动态活性部位具.
  • 在低纳米分子度下使用高亲和度捕获进行分析.
  • 开发了一个机器学习框架来解释来自单个转位事件的多状态信号.

主要成果:

  • 从单个转位事件中实现的高保真分类.
  • 在使用机器学习框架对单个事件进行分类时,证明了~91%的准确性.
  • 成功地解决了复杂的混合物,这些复杂的混合物无法通过散装聚合方法处理.

结论:

更多相关视频

High Resolution Physical Characterization of Single Metallic Nanoparticles
09:56

High Resolution Physical Characterization of Single Metallic Nanoparticles

Published on: June 28, 2019

4.9K
Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

1.6K

相关实验视频

Last Updated: Jun 19, 2026

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

14.2K
High Resolution Physical Characterization of Single Metallic Nanoparticles
09:56

High Resolution Physical Characterization of Single Metallic Nanoparticles

Published on: June 28, 2019

4.9K
Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

1.6K
  • 为真正的单分子蛋白质组学建立了一个框架.
  • 克服了纳米孔感应中集合平均化的局限性.
  • 开辟了分析单分子水平的复杂生物样本的新可能性.