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Hpo的可逆乙化调节了Hippo路径
Fengyun Lei1, Yan Ding2,3, Yanyun Liu3
1Key Laboratory of Biodiversity Conservation and Bioresource Utilization of Jiangxi Province, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China.
河马通路调节器官的大小. 这项研究表明,Nej和Hdac3对Hpo的乙化控制了其稳定性,影响了Hippo的信号传递,并为癌症等疾病提供了新的治疗点.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 发育生物学 发展生物学
背景情况:
- 河马通路对于器官大小和组织平衡至关重要.
- 河马通路的失调与包括癌症在内的疾病有关.
- 像化和无处不在化这样的翻译后修改会影响Hippo路径的组件,但乙化的作用尚不清楚.
研究的目的:
- 研究乙化在调节河马信号通路中的作用.
- 为了确定参与Hippo通路组件的乙化和脱乙化的特定酶.
- 了解这些修改如何影响通路活动和细胞过程.
主要方法:
- 研究了Hpo (Hippo通路激酶) 的乙化.
- 利用了Nej (乙转移酶) 和Hdac3 (脱乙酶) 的基因操纵 (击倒,过度表达).
- 评估了对Hippo目标基因表达和蛋白质稳定性的影响.
主要成果:
- Hpo经历由Nej和Hdac3调节的可逆乙化,影响其稳定性.
- 失去Hdac3会增加Hippo目标基因表达,这表明Hdac3在Wts.上游起作用.
- 细胞质Hdac3 deacetylates和稳定Hpo,而Nej knockdown降低了目标基因表达,通过Hdac3沉默可逆.
结论:
- 可逆乙化在控制Hippo路径方面发挥着重要作用.
- Hdac3在细胞质中去乙化和稳定Hpo,调节Hippo的信号传输.
- 研究结果提供了关于生长调节的见解,以及与河马通路相关的疾病的潜在治疗策略.
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