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酸和酸可能会损害结节上皮质屏障功能,并导致牙周炎的进展
Takako Oikawa1, Toshitaka Miura2, Ting Wang3
1Division of Periodontology, Department of Conservative Dentistry, School of Dentistry, Iwate Medical University, Morioka, Iwate, 020-8505, Japan.
Journal of oral biosciences
|February 19, 2026
概括
酸 (PA) 和酸 (AA) 通过破坏细胞循环进展和粘附分子表达来阻碍结点上皮细胞的修复和增殖. 这些发现表明多个短链脂肪酸 (SCFA) 导致牙周炎的病原性.
科学领域:
- 口腔微生物学 口腔微生物学
- 细胞生物学 细胞生物学
- 牙周病研究 牙周病研究
背景情况:
- 牙周炎是导致牙脱落的主要原因,涉及细菌毒性因素和代谢产物,如短链脂肪酸 (SCFA).
- 已知特定的SCFA,黄油酸 (BA) 和乳酸 (LA),会损害牙和结节上皮细胞功能.
- 其他SCFA,酸 (PA) 和酸 (AA) 对结节上皮的影响仍然在很大程度上未被研究.
研究的目的:
- 研究酸 (PA) 和酸 (AA) 对小鼠JE-1细胞的影响,这些细胞来自牙结膜上皮质.
- 阐明PA和AA影响表皮细胞行为与牙周炎相关的机制.
主要方法:
- 鼠JE-1细胞被暴露在PA或AA中.
- 评估结果包括伤口愈合,细胞增殖和活力.
- 量化逆转录聚合酶链反应 (qRT-PCR) 用于分析关键基因的mRNA表达.
主要成果:
- PA和AA显著延迟了伤口关闭和减少了细胞增殖,但没有影响细胞活力.
- 这两种酸都导致了整合素α6和整合素β4.4的下调.
- 细胞循环分析显示G0/G1停止,Ccnd1下降和p21表达增加.
结论:
- 酸和酸会损害结点上皮细胞的伤口修复,增殖和粘附.
- 这些效应是由细胞循环进展的改变和粘附相关基因的表达介导的.
- 多个SCFA可能导致牙周炎的上皮层屏障功能障碍,需要进一步的体内调查.
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