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Taste Exam: A Brief and Validated Test
Published on: August 17, 2018
通过TAS1Rs了解有机酸的味道
Yuko Yamase1, Katsuki Takebe2, Kengo Horie3
1Department of Dental Anesthesiology and Special Care Dentistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 700-8525, Japan; Department of Oral Physiology, Graduate School of Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan.
小鼠表现出对有机酸的反原体特异性消耗,这可能是由TAS1R3依赖的摄入后信号介导的. 这表明TAS1R3在味觉感知中的作用超出了甜味和umami.
科学领域:
- * 感官科学和味觉受体研究.
- *哺乳动物的化学感知和行为反应.
背景情况:
- *有机酸是发酵食品中的关键味道成分,有助于酸性.
- * 有机酸,如乳酸,因因子体的特异性识别表明有专门的味道路径.
- * TAS1Rs (味觉受体类型1) 是已知的甜味和乌玛米受体,但它们在有机酸识别中的作用尚未被探索.
研究的目的:
- * 调查TAS1Rs,特别是TAS1R3在小鼠对有机酸的酶因依赖性行为反应中的作用.
- * 为了确定TAS1R3是否影响有机酸反体的差异消耗.
主要方法:
- * 用小鼠进行48小时和1小时两瓶偏好测试的行为研究.
- *使用差分扫描度法 (DSF) 的质结合试验,其中包括 Tas1r2a/Tas1r3 质结合域 (LBD).
主要成果:
- *野生型小鼠在长期测试中表现出对d-酸而不是l-酸的偏好,Tas1r3淘汰赛 (KO) 小鼠没有这种偏好.
- *在短期试验中没有观察到这种反体偏好,从而最大限度地降低了摄入后的影响.
- *DSF测定没有显示测试中的有机酸与药物Tas1r2a/Tas1r3 LBD直接结合.
结论:
- *TAS1R3似乎通过摄入后的信号来调节对有机酸的反原体选择性消耗,而不是直接与味觉受体结合.
- *与TAS1Rs的结合亲和力差异可能涉及静电相互作用和orthosteric口袋内的结网络.
- * 这突出了TAS1Rs在甜味和乌玛米味道之外的复杂味觉感知中的潜在作用.
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