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对暴露于Bis-GMA的纤维细胞进行蛋白质组分析

Yohann Flottes¹, Elisabeth Dursun²

¹Paris Cité University, Inserm, UMR 1333, Oral Health, Montrouge, France; Henri Mondor Hospital, F-94000, Créteil, France; Department of Prosthodontics, Faculty of Dental Surgery, Paris Cité University, Montrouge, France.

Journal of oral biosciences

|February 19, 2026

概括

从牙科复合材料中低水平暴露于Bisphenol A-glycidyl methacrylate (Bis-GMA) 会导致剂量依赖的细胞应激和人类牙纤维细胞的蛋白质变化. 这表明潜在的风险和需要更安全的牙科材料和程序.

科学领域:

生物材料科学是生物材料的科学.
蛋白质组学是指蛋白质组学.
细胞毒理学细胞毒理学

背景情况:

牙树脂复合材料在修复性牙科中广泛使用.
双A-甘油基甲酸盐 (Bis-GMA) 是从这些材料中释放的常见单体.
了解Bis-GMA的细胞作用对于患者的安全至关重要.

研究的目的:

研究Bis-GMA对人类牙纤维细胞蛋白质组的剂量依赖作用.
为了确定受Bis-GMA暴露影响的特定蛋白质变化和细胞通路.

主要方法:

人类牙纤维细胞在72小时内暴露于不同度的Bis-GMA (0.002,0.02和0.2mM).
使用2D电泳和LC-MS/MS进行了蛋白质组分析.
鉴定出差异丰富的蛋白质,并从功能上进行了分类.

主要成果:

总共有45种不同丰富的蛋白质被确定.
低Bis-GMA度显示了适应性反应 (线粒体,细胞骨).
较高度诱导细胞应激 (核运输,新陈代谢,蛋白质稳定性破坏),可能导致细胞亡或衰老.

结论:

即使在低水平的Bis-GMA暴露,也会诱导显著的蛋白质组变化和细胞应激.
这些发现引发了人们对牙科修复中单体释放的担忧.
临床程序应尽量减少单体释放,需要更安全的替代品.

关键词:

不良影响不良影响这是一种Bis-GMA.牙科材料牙科材料一个单体的单体.蛋白质组学是指蛋白质组学.有毒性有毒性

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