鉴定Mfa5-2作为Porphyromonas gingivalis中Mfa1 fimbriae的一个结构成分
Naoyoshi Miwa1, Makoto Hirohata2, Yui Yoshimura2
1Department of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan; Department of Pediatric Dentistry, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Porphyromonas gingivalis Mfa1 fimbriae 在EM3和1439菌株中结合了Mfa5-2蛋白. 这揭示了这些重要的细菌附属体的新结构多样性.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 结构生物学 结构生物学
背景情况:
- Porphyromonas gingivalis 是一种牙周病原体.
- Mfa1 膜对于生物膜的形成和殖民是至关重要的.
- 在Mfa1 fimbriae由Mfa1,Mfa3,Mfa4和Mfa5的子单位组成.
研究的目的:
- 在P. gingivalis EM3和1439.中调查mfa5-2基因的存在.
- 确定Mfa5-2是否被纳入这些菌株中的Mfa1 fimbriae.
主要方法:
- 在P. gingivalis EM3和1439.的基因组测序.
- 使用SDS-PAGE,质谱学和免疫阻塞,对Mfa1膜片进行净化和分析.
- 通过传输电子显微镜检查手足形态.
主要成果:
- 在P. gingivalis EM3和1439.2中,发现了mfa5-2基因与mfa5-1的下游.
- Mfa5-2被确定为纯化的Mfa1 fimbriae中的~250kDa成分.
- 尽管加入了Mfa5-2,但骨架构仍然保持一致.
结论:
- 在P. gingivalis EM3和1439中,Mfa5-2被表达并集成到Mfa1 fimbriae中.
- 这种整合代表了Mfa1 fimbriae.e.的新型结构变异.
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