重复的冷和解周期对便微生物组合的稳定性的影响
Matteo Sangermani1,2, Indri Desiati3, Nicole Quattrini4
1HUNT Center for Molecular and Clinical Epidemiology, Department of Public Health and Nursing, Norwegian University of Science and Technology, Trondheim, Norway. matteo.sangermani@ntnu.no.
Scientific reports
|February 19, 2026
概括
重新分析储存的便样本是可行的,因为重复的冷解周期对肠道微生物群的完整性影响最小. 个体间的差异超过了冷解效应,支持对微生物组研究的样本重复使用.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 这是生物银行.
背景情况:
- 与新的队列研究相比,重新分析以前收集的样本可以节省时间和资源.
- 在重复结解 (FT) 周期后,人们对便微生物组的稳定性存在担忧,这可能会导致结果偏差.
- 研究FT周期对16SrRNA基因测序的影响对于可靠的微生物组分析至关重要.
研究的目的:
- 评估重复的冷解 (FT) 周期对便微生物组合的影响,通过16S rRNA基因测序测量.
- 为了确定FT周期是否会在肠道微生物组分析中引入显著的偏差.
- 为存储的便样本的可用性提供指导,这些样本经过FT循环.
主要方法:
- 使用16S rRNA基因测序研究了多达六个FT周期对便样本的影响.
- 使用差异丰度分析工具,包括MaAsLin2和ALDEx2.
- 在新鲜,未冷的样本和经过重复FT循环的样本中比较微生物组组成.
主要成果:
- 个体间的样本变化始终超过了FT周期的影响.
- 在第一个FT周期后,MaAsLin2发现了有限的微生物基因变化,在4-6周期后出现了轻微的渐进性转变.
- 更保守的ALDEx2方法在任何FT周期中都没有检测到任何显著变化.
- 前三个FT周期对整体微生物组合的影响最小.
结论:
- 支持重新使用经过一次解的储存便样本.
- 对于经过单次FT循环的样本,损害肠道微生物群完整性的风险最小.
- FT周期对微生物组分析的影响有限,特别是使用保守的统计方法.
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