在H1N1感染的初级人类气管支气管上皮细胞中的circRNA表达概况识别了在A549细胞中验证的候选免疫相关circRNAs
Kai Shen Lim1, Mathanakumara Ealam Selvan1, Chee-Kwee Ea2
1Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603, Kuala Lumpur, Malaysia.
Archives of virology
|February 19, 2026
概括
这项研究揭示了H1N1流感感染后人类呼吸道细胞中的循环RNA (circRNA) 变化. 上调的circRNAs与抗病毒反应有关,这表明它在宿主防御流感A病毒 (IAV) 中发挥了作用.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 甲型流感病毒 (IAV) 构成了严重的流行病威胁.
- 之前在IAV感染中的循环RNA (circRNA) 研究缺乏人类初级细胞数据.
- circRNAs是细胞过程和疾病的关键调节者.
研究的目的:
- 在感染H1N1 IAV的人类气管支柱上皮细胞 (HTBE) 中描述circRNA表达.
- 在人类肺上皮细胞中验证差异表达的circRNAs (A549-PB1).
- 探索circRNAs在宿主对IAV感染的反应中的作用.
主要方法:
- 感染HTBE细胞的RNA测序 (RNA-seq) 和CIRI量子分析.
- 在A549-PB1细胞中使用RNase R消化和RT-qPCR验证circRNAs.
- 生物信息分析包括功能丰富和竞争性内源RNA (ceRNA) 网络预测.
主要成果:
- 在H1N1感染后的不同时间点确定了许多差异表达的circRNAs.
- 在较晚的感染阶段 (12-24 hpi) 中,有9个circRNA被持续上调.
- 父母和DE circRNAs的向基因参与抗病毒免疫和病毒感染途径.
- 验证的circDDX58和circPML在H1N1感染和其他病毒诱导时显示上调.
- 预测circPML和circDDX58/9参与一个circRNA-miRNA-mRNA ceRNA网络.
结论:
- 这是首次报告H1N1感染的人类初级呼吸道细胞中circRNA配置文件的研究.
- 已识别的circRNAs,包括circPML和circDDX58,是宿主抗病毒反应中的潜在关键参与者.
- 这些发现突出了circRNAs作为潜在的治疗点或流感感染的生物标志物.
- circRNAs可能代表了对病毒感染的反应中保存的分子机制.
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