Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Simple cellular potts model of scratch assays on healthy and keloid fibroblasts driven by contact inhibition of locomotion.

Journal of theoretical biology·2026
Same author

Modelling and investigating memory immune responses in infectious disease. Application to influenza a virus and sars-cov-2 reinfections.

Infectious Disease Modelling·2025
Same author

Deep Learning Approaches for the Classification of Keloid Images in the Context of Malignant and Benign Skin Disorders.

Diagnostics (Basel, Switzerland)·2025
Same author

Using Approximate Bayesian Computation to Calibrate the Model Parameters Characterizing the Autoregulatory Behavior of Microvessels.

International journal for numerical methods in biomedical engineering·2025
Same author

Post-operative glioblastoma cancer cell distribution in the peritumoural oedema.

Frontiers in oncology·2024
Same author

Modeling and investigating malaria P. Falciparum and P. Vivax infections: Application to Djibouti data.

Infectious Disease Modelling·2024

相关实验视频

Updated: Jun 28, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 24, 2015

使用单细胞和空间转录组学数据对连接体动态的组织水平PDE模型进行校准.

Ali Daher1, Dumitru Trucu2, Raluca Eftimie3

  • 1Department of Mathematics, Marie and Louis Pasteur University, Besancon, France. ali.daher@umlp.fr.

NPJ systems biology and applications
|February 19, 2026
PubMed
概括

我们开发了一个计算管道,以使用转录学数据校准反应扩散模型. 这种方法集成了单细胞RNA测序和空间转录组学,用于组织中准确的细胞通信建模.

更多相关视频

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
11:52

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

相关实验视频

Last Updated: Jun 28, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 24, 2015

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
11:52

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

科学领域:

  • 计算生物学 计算生物学
  • 系统生物学 系统生物学
  • 分子生物学分子生物学

背景情况:

  • 通过连接体介导的信号传输进行细胞通信对于生理过程至关重要.
  • 反应-扩散模型描述了连接体动态,但由于有限的微环境数据,缺乏可靠的参数校准.
  • 将各种生物数据集成到机械模型中仍然是一个挑战.

研究的目的:

  • 开发一个计算管道,用于校准反应-扩散模型,使用转录学数据.
  • 为了利用单细胞RNA测序和空间转录组学来进行组织规模模型参数推断.
  • 为将现代转录学数据集成到机械模型中提供统一的框架.

主要方法:

  • 开发了一个计算管道,集成有限体积解决器,生物信息学预处理,近似贝叶斯计算 (ABC) 和基于梯度的优化.
  • 利用开源的人体皮肤数据集进行案例研究.
  • 对转化生长因子β异型的校准参数.

主要成果:

  • 成功校准了控制人类皮肤中转化生长因子β异型的参数.
  • 将推断的空间度场与局部细胞类型分布进行比较.
  • 对合成数据集的基准测试证明了将ABC与基于梯度的优化相结合的准确性和好处.

结论:

  • 单细胞RNA测序和空间转录组学为校准组织规模机械模型提供了丰富的数据来源.
  • 开发的管道为参数推理提供了一种灵活而严格的方法.
  • 这种方法增强了我们模拟细胞通信和组织动态的能力.