细胞纳米孔的超分辨率结构化照明显微镜,使用近红外光探针
Optics express
|February 20, 2026
概括
研究人员开发了一种用于近红外 (NIR) 成像的新型超高分辨率显微镜. 这种先进的系统可以在活细胞成像应用中更深入地穿透组织,并降低光毒性.
科学领域:
- 生物光子学 生物光子学
- 光学显微镜的使用方法
- 超高分辨率的成像技术
背景情况:
- 超分辨率显微镜传统上使用可见光光体.
- 近红外 (NIR) 和短波红外 (SWIR) 成像提供了诸如降低光毒性和更深层组织透等优势.
- 将超分辨率技术扩展到NIR波长对于实时生物成像非常理想.
研究的目的:
- 开发一款针对近红外 (NIR) 波长范围优化的超高分辨率结构化照明显微镜 (SR-SIM).
- 为了实现更深层的组织透,并最大限度地减少活生物样本中的光毒性.
主要方法:
- 使用迈克尔森干扰仪通过激光束干扰产生结构化照明.
- 采用双轴度镜来控制照明角度和图案间距.
- 使用反射涂层平面平行玻璃板实现相位移.
- 使用NIR增强的26兆像素科学补充金属氧化物半导体 (sCMOS) 摄像头检测到光.
主要成果:
- 通过成像低衍射极限光珠,成功地描述了NIR超分辨率显微镜的特征.
- 通过解决肝脏侧鼻内皮细胞中的细胞纳米孔来证明该系统对生物成像的能力.
- 在NIR范围内实现超高分辨率成像,克服了以前的局限性.
结论:
- 开发的NIR超分辨率结构化照明显微镜是先进的实时生物成像的强大工具.
- 这项技术将超分辨率显微镜的好处扩展到更深层组织成像,减少光毒性.
- 该系统显示出研究复杂生物环境中的细胞结构和动态的巨大潜力.
相关概念视频
Imaging Biological Samples with Optical Microscopy
9.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.1K
Super-resolution Fluorescence Microscopy
12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Overview of Electron Microscopy
11.7K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
11.7K


