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相关概念视频

Mechanisms of Heat Transfer II01:20

Mechanisms of Heat Transfer II

In convection, thermal energy is carried by the large-scale flow of matter. Ocean currents and large-scale atmospheric circulation, which result from the buoyancy of warm air and water, transfer hot air from the tropics toward the poles and cold air from the poles toward the tropics. The Earth’s rotation interacts with those flows, causing the observed eastward flow of air in the temperate zones. Convection dominates heat transfer by air, and the amount of available space for the airflow...
Energy Transfer in Chemical Reactions01:16

Energy Transfer in Chemical Reactions

Chemical reactions require sufficient energy to cause the matter to collide with enough precision and force that old chemical bonds can be broken and new ones formed. In general, kinetic energy is the form of energy powering any type of matter in motion. Imagine a person building a brick wall. The energy it takes to lift and place one brick on top of another is the kinetic energy—the energy matter possesses because of its motion. Once the wall is in place, it stores potential energy. Potential...
Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
Deactivation Processes: Jablonski Diagram01:25

Deactivation Processes: Jablonski Diagram

Luminescence, the emission of light by a substance that has absorbed energy, is a process that involves the interaction of molecules with light. The energy-level diagram, or Jablonski diagram, is a graphical representation of these interactions, illustrating the various states and transitions a molecule can undergo. In a typical Jablonski diagram, the lowest horizontal line represents the ground-state energy of the molecule, which is usually a singlet state. This state represents the energies...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...

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相关实验视频

Updated: Jun 26, 2026

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
11:46

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

Published on: May 26, 2014

生物发光中的能量转移

Shuangqi Pi1, Ya-Jun Liu1,2

  • 1Department of Chemistry, Faculty of Arts and Sciences, Center for Advanced Materials Research, Beijing Normal University, Zhuhai, China.

Luminescence : the journal of biological and chemical luminescence
|February 20, 2026
PubMed
概括

生物发光共振能量转移 (BRET) 增强了用于深层组织生物成像的光辐射. 使用光酶和光蛋白的工程BRET系统实现了更明亮的红移信号,改善了生物过程的可视化.

科学领域:

  • 生物物理学的生物物理.
  • 生物化学 生物化学
  • 生物成像是一种生物成像.

背景情况:

  • 生物发光 (BL) 提供敏感的,非侵入性的生物成像,但受到强度和短波长辐射的限制.
  • 自然生物发光生物利用发光系统和天线蛋白质发光.
  • 生物发光共振能量转移 (BRET) 灵感来自于自然系统,以克服BL的局限性.

研究的目的:

  • 审查自然生物发光系统中能量转移 (ET) 的基本机制.
  • 总结工程BRET系统对增强生物成像的最新进展.
  • 讨论开发先进BL探测器的挑战和未来方向.

主要方法:

  • 对自然生物发光机制的实验和理论研究的分析.
  • 对工程 BRET 系统的审查,将光酶与光蛋白,有机染料和纳米材料结合起来.
  • 探索实现更明亮和红移排放的策略,包括近红外II (NIR-II) 应用.

主要成果:

  • BRET系统可以克服本地BL的局限性,实现更明亮和红移光辐射.
  • 光酶与光蛋白,染料和纳米材料的合将BL扩展到NIR-II区域.
  • 工程 BRET 探测器显示了改善深层组织成像的潜力.

更多相关视频

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
05:52

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

Published on: June 28, 2018

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
07:19

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

相关实验视频

Last Updated: Jun 26, 2026

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
11:46

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

Published on: May 26, 2014

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
05:52

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

Published on: June 28, 2018

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
07:19

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

结论:

  • 工程 BRET 系统为生物成像提供了比原生生物发光显著的改进.
  • 需要进一步开发以提高BL探测器的亮度,光谱可调性和稳定性.
  • 先进的BRET探测器将使生物过程的高分辨率,深层组织成像成为可能.