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An Ex Vivo Tissue Culture Model for Fibrovascular Complications in Proliferative Diabetic Retinopathy

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Published on: January 25, 2019

使用数字光处理进行先进的体外血管壁建模,以研究高血糖驱动的细胞变化.

Ianina Pokholenko^1,2,3, Marguerite Meeremans^1,2, Sandra Van Vlierberghe¹

¹Polymer Chemistry & Biomaterials Group, Centre of Macromolecular Chemistry (CMaC), Department of Organic and Macromolecular Chemistry, Ghent University, Ghent, Belgium.

Frontiers in bioengineering and biotechnology

|February 20, 2026

概括

研究人员开发了GelMA涂层的支架,用于研究代谢综合征. 这些支架支持马类介质细胞 (MSC) 和内皮细胞 (EC),并表明高葡萄糖损害了EC的活力,模仿疾病状况.

关键词:

尿基聚乙烯糖醇以烯酸盐为基础的烯酸盐末盖.数字光处理是数字光处理.凝甲基烯基烯基基过高血糖症 (hyperglycemia) 是一种疾病.血管壁模型血管壁模型

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科学领域:

生物材料工程生物材料工程
血管生物学血管生物学
马类科学马类科学

背景情况:

代谢综合征,普遍存在于人类和马 (马类代谢综合征),涉及肥胖,胰岛素抵抗,高血压和血管变化.
对于体外血管模型来研究代谢综合征病理生理学和为这两种物种开发治疗方法,存在极大需求.

研究的目的:

使用3D打印的脚手架开发和描述一种新的体外血管壁模型.
在正常和高血糖条件下,评估这些支架与马细胞的细胞兼容性和细胞反应.

主要方法:

数字光处理 (DLP) 用于制造烯酸结尾的聚氨基聚合物前体,并使用聚乙烯糖醇骨干 (AUP2PEG) 支架.
脚手架通过UV诱导的光聚合或通过物理吸收的I型乙烯聚合物被涂上凝甲烯基 (GelMA).
在支架上培养了马类介质体细胞 (MSC) 和内皮细胞 (EC),并评估了它们的生存能力和分化潜力,特别是在高葡萄糖条件下.

主要成果:

凝MA涂层创造了一个统一的层,增强了DLP打印的AUP2PEG支架的细胞兼容性,适用于MSC和EC.
脚手架支持MSC的三线分化.
暴露在高葡萄糖条件下显著降低了EC活力,模仿了代谢综合征中出现的内皮损伤,而MSC活力基本上没有受到影响.

结论:

用GelMA涂层的DLP打印的AUP2PEG脚手架有效地支持马匹EC和MSC的生长.
开发的模型表明,高血糖条件不利于EC在支架上的生存能力,反映了代谢综合征的体内观察.