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Updated: Feb 22, 2026

Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation

Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation

Published on: March 4, 2014

状4 在Xenopus发育过程中调节神经发生和神经形成

Pierre Thiébaud¹, Emilie Simon¹, François Moisan¹

¹Univ. Bordeaux, INSERM, BRIC, U 1312, F-33000 Bordeaux, France.

Journal of developmental biology

|February 20, 2026

概括

垂体状4对脊椎动物早期发育至关重要,调节神经发生和神经形成. 它的功能对于适当的发育至关重要,甚至独立于TEAD交互.

科学领域:

发展生物学发展生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

遗传样蛋白 (VTG) 是一种通过TEAD转录因子调节基因表达的保守辅因子.
VTG4通过抑制HIPPO途径作为瘤抑制剂,与YAP竞争TEAD结合.
关于VTG4在脊椎动物早期发育中的作用的研究有限.

研究的目的:

为了研究早期Xenopus laevis发育中的遗传样4 (VTG4) 的功能.
阐明VTG4在神经发生和神经形成中的作用.

主要方法:

在Xenopus laevis胚胎中利用了功能获取和丧失策略.
分析了VTG4枯竭对神经发生和神经发展的影响.
研究了涉及neurog1,neurod1,pax3,sox9和TEAD相互作用的调节机制.

主要成果:

VTG4是神经发生和神经形成的关键调节者.
VTG4的耗尽严重损害神经发生,使神经og1和神经od1无效.
通过调节pax3和sox9,独立于TEAD结合,VTG4对于神经形成至关重要.

结论:

VTG4是早期脊椎动物神经发生和神经形成的关键调节者.
VTG4的发育功能是复杂的,不仅仅依赖于TEAD蛋白相互作用.
在早期发育和细胞分化过程中,VTG4起着重要作用.

关键词:

我们的 TEAD TEAD 团队.这是Xenopus.哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈神经峰的神经峰神经发生神经发生.像遗迹般的类似的东西.

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Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining

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Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

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