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Updated: Feb 22, 2026

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

Published on: October 30, 2021

维生素D重新编程非编码RNA网络以阻止人类巨细胞中的寨卡病毒

Julieta M Ramírez-Mejía¹, Geysson Javier Fernandez^1,2, Silvio Urcuqui-Inchima²

¹Grupo Biología y Control de Enfermedades Infecciosas, Universidad de Antioquia UdeA, Medellín 050010, Colombia.

Pathophysiology : the official journal of the International Society for Pathophysiology

|February 20, 2026

概括

维生素D通过改变非编码RNA和mRNA网络来调节巨对寨卡病毒 (ZIKV) 的反应. 这项研究确定了参与ZIKV感染和免疫调节的关键分子和途径.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
分子生物学分子生物学

背景情况:

寨卡病毒 (ZIKV) 感染带来了先天性形和神经炎症疾病的风险.
巨细胞是ZIKV的关键目标和储存库,调解抗病毒和炎症反应.
维生素D (VitD) 是一种免疫调节剂,可以增强巨细胞的抗菌活性,并调节炎症.

研究的目的:

为了研究维生素D在ZIKV感染期间对巨细胞反应的影响.
识别ZIKV感染巨细胞中VitD调节的非编码RNA (ncRNA) 和mRNA网络.
探索ZIKV感染中VitD介导的免疫调节的潜在分子机制.

主要方法:

从人类单细胞衍生巨细胞 (MDMs) 来重新分析公开的RNA-seq和miRNA-seq数据集.
MDMs被区分为有或没有VitD,并随后感染ZIKV.
进行了差异表达分析和竞争性内源RNA (ceRNA) 网络分析.

主要成果:

在ZIKV感染的MDM中,VitD治疗显著调节了65个长非编码RNA (lncRNA) 和23个微RNA (miRNA).
特定的lncRNAs (例如HSD11B1-AS1,Lnc-FOSL2) 和miRNAs (例如let-7a,miR-146a) 被确定为免疫和代谢基因的潜在调节者.
ceRNA网络分析表明SOX2-OT和SLC9A3-AS1在调节免疫控制和病毒反应途径方面的作用.
功能丰富分析突出了与新陈代谢,应激反应和细胞迁移相关的途径.

结论:

这项研究提供了在ZIKV感染期间受到VitD影响的ncRNA-mRNA网络的探索地图.
已识别的候选分子和途径需要进一步研究宿主病毒相互作用.
突出了VitD在调节巨细胞反应和针对ZIKV的免疫调节方面的潜力.

关键词:

抗病毒反应的抗病毒反应.生物信息学是一种生物信息学.竞争的内源性RNA.在ncRNA中,我们可以它们是mRNARNA.这是一个小RNARNA.这是一种促炎性反应.病毒病毒病毒病毒

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