平衡性能和可持续性:白色差异脉冲电压测量方法用于血中甲福明和empagliflozin
Osama I Abdel Sattar1, Hamed H M Abuseada1, Mohamed S Emara1
1Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Nasr City, Egypt.
Bioanalysis
|February 20, 2026
概括
一种新的白色/绿色分析化学方法允许同时测量甲 (MEF) 和格利弗洛辛 (EMP) 在血中. 这种可持续的方法为治疗药物监测提供了一个更绿色的替代方案.
科学领域:
- 分析化学 分析化学
- 电化学 电化学 电化学
- 绿色化学 绿色化学
背景情况:
- 在血中同时监测甲胺 (MEF) 和empagliflozin (EMP) 的治疗药物至关重要.
- 现有的分析方法缺乏可持续性和绿色性,通常需要复杂的电极修改,并显示出有限的等离子体适用性.
研究的目的:
- 开发一种可持续和绿色的分析化学方法,用于在人体血中同时量化MEF和EMP.
- 验证开发的方法的准确性,精度,线性和灵敏度.
主要方法:
- 使用未经修改的玻璃碳电极 (GCE) 进行微分脉冲电压测量 (DPV).
- 样本准备涉及使用来自人体血的乙尼特进行蛋白质沉.
- 方法优化侧重于酸盐缓冲器pH值和扫描速率,随后根据ICH Q2 (R1) 准则进行验证.
主要成果:
- 该DPV方法证明了MEF (20-60μM,r=0.997) 和EMP (10-70μM,r=0.997) 两种方法的优异线性.
- 实现了低检测极限 (LOD):MEF为4.38微米,EMP为4.15微米.
- 报告了高血恢复率 (MEF为99.4%,EMP为100.4%) 和优异的绿色 (83/100) 和白色 (97/100) 分数.
结论:
- 经过验证的DPV-GCE方法为MEF和EMP在血中的量化提供了一个快速,可持续和绿色的方法.
- 这种方法作为常规治疗药物监测的概念验证,尽管临床翻译需要患者样本.
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