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相关概念视频

Rab Cascades01:25

Rab Cascades

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Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
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Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

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Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
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Coat Assembly and GTPases01:33

Coat Assembly and GTPases

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Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
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Rab Proteins01:14

Rab Proteins

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Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
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Golgi Apparatus01:49

Golgi Apparatus

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As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
105.3K
Golgi Apparatus01:09

Golgi Apparatus

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Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
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相关实验视频

Updated: Feb 22, 2026

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
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Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

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在Golgi的ARFGTPase功能.

Petia Adarska1, Luis Wong-Dilworth1, Francesca Bottanelli2

  • 1Institute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.

Sub-cellular biochemistry
|February 20, 2026
PubMed
概括
此摘要是机器生成的。

ADP-ribosylation因子 (ARFs) 是小型GTPases,它们调节细胞内运输. 它们的GDP-GTP周期由GEF和GAP控制,对于膜贩运和货物选择至关重要.

关键词:
在ARF GTPases中,国内生产总值 - GTP 交换因素激活GTP酶的蛋白质戈尔吉走私的贩运活动

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Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
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Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

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Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein
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相关实验视频

Last Updated: Feb 22, 2026

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
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Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
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科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 生物化学 生物化学

背景情况:

  • ADP-ribosylation因子 (ARFs) 是小的GTPases,对于真核细胞中细胞内运输至关重要.
  • ARFs通过在GDP-bound无活跃状态和GTP-bound活跃状态之间循环调节膜贩运.
  • 它们的功能与脂质信号通路和外套蛋白质的招募密切相关.

研究的目的:

  • 阐明ARF GTPases在细胞内运输中的调节机制.
  • 突出GDP-GTP周期在ARF激活和膜协会中的作用.
  • 在货物选择中强调ARF,脂质信号传递和外套蛋白质复合体之间的相互作用.

主要方法:

  • 该研究的重点是ARF的功能周期,涉及关氨酸核酸交换因子 (GEF) 和GTPase激活蛋白 (GAP).
  • 它检查了控制ARF膜招募和解离的分子相互作用.
  • 该研究将ARF结构,功能和调节的知识与细胞贩运途径的背景相结合.

主要成果:

  • ARF激活通过GEF介导的GDP-to-GTP交换发生,促进膜协会.
  • 通过GAP刺激的GTP水解来调节ARF无活化,从而导致膜解离.
  • 这种动态循环对于招募特定的货物适配器和外套蛋白质至关重要,从而指导囊泡形成和货物分类.

结论:

  • ARFs的GDP-GTP循环是控制细胞内膜贩运的基本机制.
  • ARFs充当关键的分子开关,整合来自脂质修饰和蛋白质相互作用的信号.
  • 了解ARF法规,可以了解货物选择和维持蜂组织的复杂过程.