隔离植物衍生细胞外囊泡的协议
Yunyi Chen1, Le Xu1, Yuanyuan Yu2
1Department of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, China.
STAR protocols
|February 20, 2026
概括
我们开发了一个强大的协议,以从各种植物来源中分离植物衍生的细胞外囊泡 (PEVs). 这种方法确保PEV的高纯度和高效回收,以便进一步研究.
科学领域:
- 植物生物学 植物生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 植物衍生的细胞外囊泡 (PEVs) 对于细胞间通信至关重要.
- 现有的隔离方法往往缺乏可重现性和效率.
研究的目的:
- 建立一个全面和可重复的协议,以从各种植物物种中分离PEV.
- 确保PEVs的高效丰富和净化.
主要方法:
- 不同离心法离心法差异
- 超过的过方法
- 通过超离心法进行超离心.
- 糖密度梯度离心 离心法 糖密度梯度离心法
主要成果:
- 成功地从各种植物来源中分离和净化PEV.
- 实现了有效的细胞碎片的去除.
- 以形态,尺寸分布和泽塔潜力为特征的PEV.
结论:
- 开发的协议为PEV隔离提供了一种可靠的方法.
- 这种方法有助于进一步研究PEV在植物中的功能.
相关概念视频
Contact-dependent Signaling
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
Gap Junctions
In animal cells, gap junctions are formed...
Plasmodesmata
The organs in a multicellular organism’s body are made up of tissues formed by cells. To work together cohesively, cells must communicate. One way that cells communicate is through direct contact with other cells. The points of contact that connect adjacent cells are called intercellular junctions.Intercellular junctions are a feature of fungal, plant, and animal cells alike. However, different types of junctions are found in different kinds of cells. Intercellular junctions found in animal...
The Apoplast and Symplast
Plant growth depends on its ability to take up water and dissolved minerals from the soil. The root system of every plant is equipped with the necessary tissues to facilitate the entry of water and solutes. The plant tissues involved in the transport of water and minerals have two major compartments - the apoplast and the symplast. The apoplast includes everything outside the plasma membrane of living cells and consists of cell walls, extracellular spaces, xylem, phloem, and tracheids. The...
Gap Junctions
The cytoplasm of adjacent animal cells can exchange small molecules, ions, and secondary messengers via the communication channels which form the gap junctions. These junctions comprise a few hundred to thousands of molecular channels, each made of two halves, called the connexon hemichannel. A connexon is a hexamer of six transmembrane connexin proteins, which assemble radially, thus forming a pore or channel in the center. One connexon hemichannel docks with a corresponding connexon on the...
Plasmodesmata
In a multicellular organism, cells must communicate to work together in a coordinated manner. One way that cells communicate is through direct contact with other cells. The points of contact that connect adjacent cells are called intercellular junctions.
Intercellular junctions are a feature of fungal, plant, and animal cells. However, different types of junctions are found in different kinds of cells. Intercellular junctions found in animal cells include tight junctions, gap junctions, and...
Intercellular junctions are a feature of fungal, plant, and animal cells. However, different types of junctions are found in different kinds of cells. Intercellular junctions found in animal cells include tight junctions, gap junctions, and...


