Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Whole-mouse immunolabeling at cellular resolution for comprehensive 3D atlases.

Nature protocols·2026

Same author

Pathogenic role of serpin B3-positive neutrophils in reinforcing thrombus stiffening during ischemic stroke.

Blood·2026

Same author

Cross-kingdom miRNA delivery by Panax notoginseng-derived extracellular-like nanoparticles vesicles restores neuronal function after ischemic injury.

Journal of nanobiotechnology·2026

Same author

Penumbra-derived small extracellular vesicles carry pathogenic RNA driving remote damage after cortical infarction.

Brain : a journal of neurology·2026

Same author

IGF2BP1 positively regulates CircOGDH accumulation in hypoxia induced stress granules.

Acta pharmaceutica Sinica. B·2026

Same author

Ultraviolet-tyrosinase cascade caged antisense oligonucleotide for precise treatment of hyperpigmentation.

Journal of controlled release : official journal of the Controlled Release Society·2025

Same journal

Computational protocol for modeling supported nanoparticle catalysts with strong metal-support interactions.

STAR protocols·2026

Same journal

Protocol for genomic mapping of chromatin targets using high-throughput CUT&RUN.

STAR protocols·2026

Same journal

A protocol for noninvasive quantification of dietary fat absorption in mice.

STAR protocols·2026

Same journal

Protocol for an AlCl<sub>3</sub>-induced Alzheimer's model in zebrafish larvae with optimized pH and behavioral assessment.

STAR protocols·2026

Same journal

Protocol for live cell barcoding and immunophenotyping of human hematological malignancies using cytometry by time of flight.

STAR protocols·2026

Same journal

Generating drug resistance models in human and murine cancer cell lines and assessing cross-resistance to chemotherapeutics and KRAS inhibitors.

STAR protocols·2026

查看所有相关文章

Search research articles

相关实验视频

Updated: Feb 22, 2026

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

隔离植物衍生细胞外囊泡的协议.

Yunyi Chen¹, Le Xu¹, Yuanyuan Yu²

¹Department of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, China.

|February 20, 2026

概括

此摘要是机器生成的。

我们开发了一个强大的协议,以从各种植物来源中分离植物衍生的细胞外囊泡 (PEVs). 这种方法确保PEV的高纯度和高效回收,以便进一步研究.

关键词:

生物技术和生物工程细胞生物学细胞生物学植物科学植物科学

更多相关视频

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Published on: February 3, 2022

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

相关实验视频

Last Updated: Feb 22, 2026

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Published on: February 3, 2022

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

科学领域:

植物生物学植物生物学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

植物衍生的细胞外囊泡 (PEVs) 对于细胞间通信至关重要.
现有的隔离方法往往缺乏可重现性和效率.

研究的目的:

建立一个全面和可重复的协议,以从各种植物物种中分离PEV.
确保PEVs的高效丰富和净化.

主要方法:

不同离心法离心法差异
超过的过方法
通过超离心法进行超离心.
糖密度梯度离心离心法糖密度梯度离心法

主要成果:

成功地从各种植物来源中分离和净化PEV.
实现了有效的细胞碎片的去除.
以形态,尺寸分布和泽塔潜力为特征的PEV.

结论:

开发的协议为PEV隔离提供了一种可靠的方法.
这种方法有助于进一步研究PEV在植物中的功能.