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创建高质量的45色光谱流细胞计数据的协议,用于无监督的聚类,以研究人类PBMCs的衰老

Claudia J Krause1, Akihide Higuchi2, Shinji Tashiro1

  • 1Life Science Technology Research & Development Department, Technology Development Laboratories, Sony Corporation, Tokyo 141-8610, Japan.

STAR protocols
|February 20, 2026
PubMed
概括

本研究介绍了使用45色面板进行高参数光谱流细胞计的协议. 该方法有助于识别人体外围血液细胞中因年龄而产生的差异.

关键词:
生物信息学是一种生物信息学.细胞生物学 细胞生物学流动细胞计量 (Flow Cytometry) 是一种流动细胞计.免疫学 免疫学 免疫学

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相关概念视频

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...