通过在小鼠中重复暴露来建模先天免疫训练的协议 (辅助调节)
Thais Boccia1, Matheus Deroco Veloso da Silva2, Victor Fattori3
1Department of Surgery, Boston Children's Hospital - Harvard Medical School, Boston, MA 02115, USA.
STAR protocols
|February 20, 2026
概括
这项研究详细介绍了一种小鼠模型协议,用于通过暴露来研究先天免疫训练和重编程. 它允许在各种条件下评估免疫调节细胞,并且对人类细胞具有翻译相关性.
科学领域:
- 免疫学 免疫学 免疫学
- 具有天生的免疫力.
- 实验模型 实验模型
背景情况:
- 天生的免疫训练和重编程对于免疫反应至关重要.
- 暴露是一种常见的辅助剂,用于疫苗.
- 了解免疫调节细胞种群是调节免疫反应的关键.
研究的目的:
- 介绍一个全面的协议,以建模先天免疫训练和重编程在小鼠.
- 为了能够在稳定状态和炎症条件下评估免疫调节细胞群.
- 利用人类细胞探索这些发现的翻译相关性.
主要方法:
- 开发一种小鼠模型,涉及重复暴露于.
- 采用细胞转移,体内抗原特异性免疫接种和移植模型的整合.
- 在体外刺激人类外周血液单核细胞 (PBMCs).
主要成果:
- 该协议允许详细建模天生的免疫训练和重编程.
- 它有助于评估免疫调节细胞动态.
- 这项研究为转化研究提供了一个框架,将小鼠模型与人类细胞反应联系起来.
结论:
- 该协议提供了一种强大的方法来研究先天免疫记忆和可塑性.
- 它支持在不同的生理和病理背景下研究免疫调节细胞.
- 该方法适用于临床前研究和翻译研究.
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